[Arabidopsis] medium-throughout Arabidopsis genotyping solution?

Forde, Brian via arab-gen%40net.bio.net (by b.g.forde from lancaster.ac.uk)
Wed Feb 6 06:15:53 EST 2008


Hi Dimitry

The following paper by Katie Clark and Patrick Krysan is Open Access and
describes a protocol that might be suitable for your purposes:

Protocol: An improved high-throughput method for generating tissue
samples in 96-well format for plant genotyping (Ice-Cap 2.0)
http://www.plantmethods.com/content/3/1/8

Hope this helps

Brian


-----Original Message-----
From: arab-gen-bounces from oat.bio.indiana.edu
[mailto:arab-gen-bounces from oat.bio.indiana.edu] On Behalf Of Jai S Rohila
Sent: 05 February 2008 14:57
To: Belostotsky, Dmitry
Cc: arab-gen from magpie.bio.indiana.edu
Subject: Re: [Arabidopsis] medium-throughout Arabidopsis genotyping
solution?


Hi Dmitry,

Try the following protocol.

High-Throughput DNA Extraction Method Suitable for PCR
Zhanguo Xin, Jeff P. Velten, Melvin J. Oliver, and John J. Burke
BioTechniques Vol. 34, No. 4: pp 820-826 (Apr 2003)
<http://www.biotechniques.com/default.asp?page=article_archive&subsectio
n=listing&year=2003&issue=4/1/article.asp?id=4120037>Full 
text

Best,

Jai


At 05:41 PM 2/4/2008, Belostotsky, Dmitry wrote:
>Anyone can recommend a method that can be used in 96 well plate or
>similar format WITHOUT robotics - and produce reliable shorty 
>quality PCRable stuff?
>Thanks much
>
>Dmitry
>
>Dmitry A. Belostotsky, Ph.D.
>
>Associate Professor
>
>Division of Molecular Biology and Biochemistry
>School of Biological Sciences
>University of Missouri
>Kansas City, MO 64110
>
>
>
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