[Arabidopsis] double selection: sulfadiazine and kanamycin

Lee Hunt via arab-gen%40net.bio.net (by l.hunt from sheffield.ac.uk)
Wed Feb 20 12:15:27 EST 2008


Hi Tine

I've not had any problem doing this, as long as the kanamycin isn't silenced in
one of the parents (I assume this is a result of crossing). I used 5mg/L sulf,
50mg/L kan. The plants do struggle and take much longer than normal to recover
as they would whichever two antibiotics you used

Lee



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