[Arabidopsis] sample drifting in live cell imaging.
(by xu.161 from osu.edu)
Thu Jan 17 13:48:01 EST 2008
Recently I has been imaging live Arabidopsis root tip cells with a
inverted Leica TCS SL confocal. I germinate seedlings on labTek II
chambered coverglass system. Unfortunately my samples keep drifting
about 0.5um/min during imaging. I think I have secured the stage so
that the drifting likely comes from the movement of the roots.
Anybody experienced similar problems? Any suggestions would be greatly
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