From sugimoto from psc.riken.jp Mon Mar 3 01:05:52 2008 From: sugimoto from psc.riken.jp (Keiko Sugimoto) Date: Mon Mar 3 12:11:45 2008 Subject: [Arabidopsis] postdoc position at RIKEN Plant Science Center Message-ID: <002b01c87cf4$a3035c30$e90a1490$@riken.jp> A Postdoctoral Scientist Position at the Plant Sciences Center, RIKEN Yokohama Institute A postdoctoral scientist position is available from April 2008 to study the size and ploidy control in plants. The successful candidate will characterize novel loss-of-function or gain-of-function mutants in Arabidopsis that display various developmental defects associated with ploidy. The research will be conducted in Keiko Sugimoto's lab (http://labs.psc.riken.jp/cfru/English/index.html) at the RIKEN Plant Science Center situated in Yokohama, Japan. RIKEN Plant Science Center has state-of-the-art facilities for genome, proteome and metabolome research. Applicants should have a PhD in plant molecular biology, developmental genetics, or cell biology. Experience in Arabidopsis molecular genetics or cell biology is highly desirable. This post is for up to 3 years, pending on the annual assessment. Please visit the RIKEN website for further detail. (http://www.riken.jp/engn/r-world/info/recruit/080228.html) Applicants are also encouraged to seek for their own funding through, for example, the RIKEN postdoctoral fellowship program, JSPS postdoctoral fellowship program, and human frontier science program. Please send a curriculum vitae, a brief summary of previous research, a statement of research interests and a letter of reference to: Keiko Sugimoto Cell Function Research Unit, Plant Sciences Center, RIKEN 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 JAPAN Tel: +81-45-503-9575 Fax: +81-45-503-9591 e-mail: sugimoto@psc.riken.jp ------------------------------------------------------------------ Keiko Sugimoto Group Leader Plant Science Center RIKEN Yokohama Institute 1-7-22 Tsurumi, Yokohama, Kanagawa, 230-0045, Japan Tel: +81-45-503-9575 Fax: +81-45-503-9591 http://www.psc.riken.jp/eng/group/index.html ------------------------------------------------------------------- From nsiefers from ou.edu Mon Mar 3 16:06:35 2008 From: nsiefers from ou.edu (Siefers, Nicholas A.) Date: Tue Mar 4 02:10:08 2008 Subject: [Arabidopsis] Quantification of total chlorophyll; chlorophyll a and b Message-ID: Dear Arab-gen, We are looking for alternative methods of quantifying total chlorophyll and/or chlorphyll a and b. Presently we are using the dimethyl formamide methods as desribed in the two papers: Moran, 1982, Plant Phys, 69:1376 Inskeep 1985, Plant Phys, 77:483 If your lab is using an alternative method for quantification of chlorophyll please notify me via email at: nsiefers@ou.edu thanks in advance, Nicholas Siefers Holt Lab University of Oklahoma From keith.schulze from anu.edu.au Mon Mar 3 23:22:54 2008 From: keith.schulze from anu.edu.au (Keith Schulze) Date: Tue Mar 4 02:10:17 2008 Subject: [Arabidopsis] Arabidopsis 35s::GUS lines Message-ID: Dear all, I was wondering if anybody might be able to share some 35s::GUS Arabidopsis lines (Col-0 background preferably). The selection marker for the GUS cassette needs to be something other than Kanamycin. Many thanks in advance. Kind regards, Keith Keith Schulze PhD Student Environmental Biology Group Research School of Biological Science The Australian National University GPO Box 475 Canberra ACT 2601 Australia Ph(work): +61 2 612-52406 Ph(mob): +61 42 080-5672 From ejr24 from cornell.edu Tue Mar 4 13:58:06 2008 From: ejr24 from cornell.edu (Edwin J. Reidel) Date: Tue Mar 4 23:38:06 2008 Subject: [Arabidopsis] Re: Quantification of chlorophyll In-Reply-To: <200803041703.m24H3cL06838@net.bio.net> References: <200803041703.m24H3cL06838@net.bio.net> Message-ID: <47CD9BBE.90208@cornell.edu> Dear Nicholas, I recently needed to quantify chlorophylls and found that most researchers continue to use the original method of Arnon (Arnon, D.I. (1949) Plant Physiol. 24, 1–15). However, subsequent studies indicate inaccuracies in his extinction coefficients. I decided to use the highly-cited method of Porra et al. (Biochimica et Biophysica Acta (BBA) - Bioenergetics. Volume 975, Issue 3, August 1989, Pages 384-394). The equations also appear in a review by Porra (PHOTOSYNTHESIS RESEARCH Volume: 73 Issue: 1-3 Pages: 149-156). They present updated equations for quantifying chlorophyll a, b, and a + b in three solvents: buffered aqueous 80% acetone, DMF, and methanol. I chose 80% acetone. Best regards, Edwin Reidel Postdoctoral Research Associate Turgeon Lab Cornell University arab-gen-request@oat.bio.indiana.edu wrote: > ------------------------------ > > Message: 2 > Date: Mon, 3 Mar 2008 15:06:35 -0600 > From: "Siefers, Nicholas A." > Subject: [Arabidopsis] Quantification of total chlorophyll; > chlorophyll a and b > To: "arab-gen@net.bio.net" > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Dear Arab-gen, > > We are looking for alternative methods of quantifying total chlorophyll and/or chlorphyll a and b. Presently we are using the dimethyl formamide methods as desribed in the two papers: > > Moran, 1982, Plant Phys, 69:1376 > > Inskeep 1985, Plant Phys, 77:483 > > If your lab is using an alternative method for quantification of chlorophyll please notify me via email at: > nsiefers@ou.edu > > thanks in advance, > Nicholas Siefers > Holt Lab > University of Oklahoma From zhangjane2001 from gmail.com Tue Mar 4 22:01:23 2008 From: zhangjane2001 from gmail.com (Jian Zhang) Date: Tue Mar 4 23:38:14 2008 Subject: [Arabidopsis] lignin quantification Message-ID: <000001c87e6d$3327cac0$99776040$@com> Hi, everyone, I want to measure the lignin content in rice, most of the publications used the method of [Kirk, T.K., and Obst, J.R. (1988). Lignin determination. Methods Enzymol. 161, 87-101.]. but this reference is too old to be available. Is there anyone have this protocol or any alternative method? You will be greatly appreciated for any of your information and suggestions. Thanks! Jian From cohen047 from umn.edu Tue Mar 4 23:58:31 2008 From: cohen047 from umn.edu (cohen047@umn.edu) Date: Wed Mar 5 01:11:30 2008 Subject: [Arabidopsis] lignin quantification In-Reply-To: <000001c87e6d$3327cac0$99776040$@com> References: <000001c87e6d$3327cac0$99776040$@com> Message-ID: Jian: Methods in Enzymology is available online back to volume 1 via Science Direct http://www.sciencedirect.com/science/bookseries/00766879 However, there are other papers that may be of interest (although you may consider these old as well!), my favorite is from George Buta, my former colleague at the USDA: Buta et al. FT-IR determination of lignin degradation in wheat straw by white rot fungus Stropharia...J Ag Food Chem 37:1382 1989 I hope this helps...Jerry On Mar 4 2008, Jian Zhang wrote: >Hi, everyone, > > > > I want to measure the lignin content in rice, most of the publications > used the method of [Kirk, T.K., and Obst, J.R. (1988). Lignin > determination. Methods Enzymol. 161, 87-101.]. but this reference is too > old to be available. Is there anyone have this protocol or any > alternative method? You will be greatly appreciated for any of your > information and suggestions. Thanks! > > > > > >Jian > > > >_______________________________________________ >Arab-gen mailing list >Arab-gen@net.bio.net >http://www.bio.net/biomail/listinfo/arab-gen > From kinoue from ucdavis.edu Wed Mar 5 15:01:45 2008 From: kinoue from ucdavis.edu (Kentaro Inoue) Date: Wed Mar 5 23:22:06 2008 Subject: [Arabidopsis] A postdoc position in UCDavis Message-ID: A postdoctoral position is available in UCDavis starting on or after May 1st, 2008, to study mechanism and significance of protein maturation and targeting in the biogenesis of photosynthetic compartments in higher plants. A number of proteins involved in photosynthetic light reaction are targeted to thylakoid lumen with signal peptides that are cleaved by thylakoidal processing peptidase (TPP). There are at least three TPP homologs in the model plant Arabidopsis thaliana. Recently, we took a genetic approach to show that one of them called Plsp1 is essential for proper thylakoid development (J Cell Biol '05 171, 425-430). Interestingly, Plsp1 is located in both thylakoids and the envelope of the organelle, whereas other two TPP homologs appear to exist only in thylakoids. The successful candidate for this position is expected to use the reagents generated in the previous study and also to develop new tools to address the significance of gene duplication giving rise to multiple TPPs, and/or the mechanism of intraorganellar protein sorting. A strong background in molecular biology and biochemistry is required. Experience of molecular cloning, over-expression of a gene of interest in bacteria and/or other organisms, and genetic analysis of model organisms is also desirable. The initial appointment is for 1-2 years and its renewal is highly possible, depending on the performance of the individual. To apply, please send a full C.V. including a brief description of research experience and interests, and names/email addresses of three references via email to: Kentaro Inoue Assistant Professor Department of Plant Sciences University of California One Shields Avenue, Davis, CA 95616, USA kinoue@ucdavis.edu From horvathdp from gmail.com Thu Mar 6 22:07:24 2008 From: horvathdp from gmail.com (David Horvath) Date: Thu Mar 6 22:26:29 2008 Subject: [Arabidopsis] ems mutagenized population? Message-ID: Hi, I am looking for a population of EMS mutagenized seeds. I looked on TAIR to see if such a thing was available, but didn't find one. Does anyone out here have a population of EMS mutagenized seeds they could share or know where I might obtain one? Dave Horvath USDA/ARS Fargo ND From Yves.Poirier from unil.ch Thu Mar 6 06:58:36 2008 From: Yves.Poirier from unil.ch (Yves Poirier) Date: Thu Mar 6 22:26:49 2008 Subject: [Arabidopsis] postdoc position University of Lausanne, Switzerland Message-ID: A postdoctoral position is available to study the carbon flux through the early steps of the isoprenoid pathway in Arabidopsis thaliana. This research program is part of a European consortium funded in FP7 that aims at developing alternative crop plants for the production of natural rubber. The ideal candidate will be experienced in plant molecular biology and/or plant biochemistry. The position is available for a maximum of 4 years, starting May 1st 2008 or soon thereafter. Applications including a full CV, list of publications, outline of research interest and names of two referees should be sent to Prof. Yves Poirier, Department of Plant Molecular Biology, Biophore Building, University of Lausanne, CH-1015 Lausanne, or by email to yves.poirier@unil.ch. To get more information on the laboratory and the Department, visit the web site at http://www.unil.ch/dbmv/page8008_en.html. -- Prof. Yves Poirier D?partement de Biologie Mol?culaire V?g?tale Le Biophore Quartier Sorge Universit? de Lausanne CH-1015 Lausanne Switzerland tel: (41)(21) 692 42 22 fax: (41)(21) 692 41 95 From cohen047 from umn.edu Fri Mar 7 00:02:12 2008 From: cohen047 from umn.edu (Jerry D. Cohen) Date: Fri Mar 7 11:45:03 2008 Subject: [Arabidopsis] ems mutagenized population? In-Reply-To: References: Message-ID: <47D0CC54.1040300@umn.edu> You can get these from Lehle Seeds: http://www.arabidopsis.com/main/cat/seeds/M2/EMS/!2e.html They also have Gamma-ray and Fast neutron M2 populations as well. I hope this helps, Jerry Cohen David Horvath wrote: > Hi, > > I am looking for a population of EMS mutagenized seeds. I looked on TAIR to > see if such a thing was available, but didn't find one. Does anyone out here > have a population of EMS mutagenized seeds they could share or know where I > might obtain one? > > Dave Horvath > USDA/ARS > Fargo ND > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > -- ?????? From hvs from life.ku.dk Fri Mar 7 11:46:42 2008 From: hvs from life.ku.dk (Henrik Vibe Scheller) Date: Fri Mar 7 18:55:55 2008 Subject: [Arabidopsis] ems mutagenized population? In-Reply-To: References: Message-ID: <47D17F82020000460001CE18@gwmta1.kvl.dk> EMS mutagenized seeds are available from Lehle Seeds http://www.arabidopsis.com Best wishes Henrik Scheller Joint Bioenergy Institute Berkeley >>> "David Horvath" 3/6/2008 19:07 >>> Hi, I am looking for a population of EMS mutagenized seeds. I looked on TAIR to see if such a thing was available, but didn't find one. Does anyone out here have a population of EMS mutagenized seeds they could share or know where I might obtain one? Dave Horvath USDA/ARS Fargo ND _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen From tberardi from acoma.stanford.edu Fri Mar 7 17:14:03 2008 From: tberardi from acoma.stanford.edu (Tanya Berardini) Date: Fri Mar 7 18:56:01 2008 Subject: [Arabidopsis] TAIR is looking for new curators! Message-ID: <8e22ab960803071414l3f082510l3072bb72f0fdd596@mail.gmail.com> Two positions available, starting March - May 2008. TAIR, The Arabidopsis Information Resource (http://arabidopsis.org) is a National Science Foundation-funded web resource for thousands of plant biology researchers worldwide, providing free access to a wide array of biological data and tools for the model plant Arabidopsis. The Carnegie Institution Department of Plant Biology is co-located with the Department of Global Ecology on a seven-acre site on the campus of Stanford University. (1) Literature annotation curator We are currently seeking applicants for the position of scientific curator to join us in our functional annotation effort. The successful candidate will work with a team of curators, software engineers, and database developers to provide data from the experimental primary literature to over 13,000 users of the TAIR website and database. The main responsibilities of this position include reading current research articles to extract gene function and other attributes for loading into the TAIR database, answering questions from the community on how to use the site, presenting information on how to use TAIR at meetings and workshops, assisting scientists with large data submissions, assisting with the design of new or improved web interfaces and tools, and participating in the development of controlled vocabulary terms for gene annotation. The ideal candidate will have a Ph.D in Biology or related discipline with research experience in plant biology, a demonstrated ability for independent, critical thinking, excellent written and verbal communication skills, the ability to work well as part of a team and good basic computer skills. Experience with Internet-based literature searching, Unix, HTML and mySQL would be useful but is not required. Qualified candidates should email a cover letter, CV and names of 3 references to Dr. Tanya Berardini at: tberardini@arabidopsis.org . (2)Genome Annotation Curator We are currently seeking applicants for the position of scientific curator to work on our genome annotation project. The successful candidate will be involved in computational and manual updates of structural annotation for genes and other sequenced objects and computational updates of functional annotation. Duties include: analyzing and interpreting sequence data, comparative genomics techniques, examining experimental data and compiling results; dealing with user requests and presenting and/or publishing data. You will also work alongside our technical team to assist with the design of new or improved web interfaces and tools. The ideal candidate will have a PhD in biology, molecular biology or related field, a demonstrated ability for independent critical thinking, knowledge of sequence analysis and annotation approaches and concepts, good communication skills and a keen eye for detail. Familiarity with UNIX, HTML, MYSQL and Perl would be beneficial but is not required. Qualified candidates should email a cover letter, CV and names of 3 referees to Dr. David Swarbreck at: dswarbreck@stanford.edu These are both on-site positions, telecommuters will not be considered. The Carnegie Institution for Science is an equal opportunity employer. From jrunions from brookes.ac.uk Tue Mar 11 03:24:26 2008 From: jrunions from brookes.ac.uk (John Runions) Date: Tue Mar 11 13:46:57 2008 Subject: [Arabidopsis] Postdoctoral position at Oxford Brookes Message-ID: <47D641BA.9070403@brookes.ac.uk> Oxford Brookes University's School of Life Sciences (oxford, UK) has a Post-Doctoral Research Assistant position available in the laboratory of Dr. John Runions. The position is funded by BBSRC for 3 years to study interactions between proteins of the plasma membrane and the actin cytoskeleton in plant cells. Recent reports suggest that cell surface proteins can physically interact with the actin cytoskeleton. These interactions are potentially important in cell signalling and in structuring the plasma membrane. The successful applicant will evaluate the role of actin cytoskeleton in membrane protein dynamics using advanced confocal microscopy techniques such as photoactivation of green fluorescent protein and motion tracking in timelapse images. See: Runions et al., Photoactivation of GFP reveals protein dynamics within the endoplasmic reticulum membrane. Journal of Experimental Botany. 57: 43-50. As well as confocal microscopy, main duties of the position will include; construction of molecular biological vectors for plant-cell transformation , analysis of protein dynamics using software such as Volocity and ImageJ, and some mathematical modelling using the protein dynamics data. The position requires a PhD in molecular biology or biochemistry and preference will be given to those that have experience in plant cell biology and confocal microscopy. You should be able to work independently and should possess good organisational and communication skills. Oxford Brookes University is situated in Oxford, England. The area has become a centre for biotechnological excellence. Bioimaging is one of the strengths of the School of Life Sciences and the Plant Cell Biology group has an excellent track record. We have two Zeiss confocal microscopes as well as TEM and SEM. Please see our website: [1]http://www.brookes.ac.uk/lifesci/research/groups/plantcellbiology Please address any informal enquiries to Dr. John Runions ([2]jrunions@brookes.ac.uk). You can download a job application package at: [3]https://edm.brookes.ac.uk/hr/hr/vacancies.do?id=5127194 -- ********************************* C. John Runions, Ph.D. School of Life Sciences Oxford Brookes University Oxford, UK OX3 0BP email: [4]jrunions@brookes.ac.uk phone: +44 (0) 1865 483 964 Runions' lab web site Visit The Illuminated Plant Cell dot com Oxford Brookes Master's in Bioimaging with Molecular Technology References 1. http://www.brookes.ac.uk/lifesci/research/groups/plantcellbiology 2. mailto:jrunions@brookes.ac.uk 3. https://edm.brookes.ac.uk/hr/hr/vacancies.do?id=5127194 4. mailto:jrunions@brookes.ac.uk From ctellison from ucdavis.edu Wed Mar 12 13:47:19 2008 From: ctellison from ucdavis.edu (Cory Ellison) Date: Wed Mar 12 15:55:31 2008 Subject: [Arabidopsis] GAL4 DBD Antibody Message-ID: <200803121847.m2CIlJ78017115@biglossa.ucdavis.edu> Hi Everyone, Does anyone have any experience with the Santa Cruz Biotech monoclonal GAL4 DNA Binding Domain antibody? I've been trying to use this in my Westerns against recombinant protein in yeast and have been unsuccessful. I have been extracting total crude protein from 5 mL yeast cultures at OD600 1.0 and still can't see anything on my Westerns. Is there a special protocol for this antibody? Is it just not very good/stable? From staceyg from missouri.edu Wed Mar 12 16:47:30 2008 From: staceyg from missouri.edu (Stacey, Gary) Date: Wed Mar 12 17:45:48 2008 Subject: [Arabidopsis] FW: DOE Positions open Message-ID: <5823E86B5049E24CAE8B2203D93EBC760386EE94@UM-XMAIL07.um.umsystem.edu> Note job ad below Gary Stacey, Ph.D. National Center for Soybean Biotechnology Divisions of Plant Sciences and Biochemistry Department of Molecular Microbiology and Immunology 271E Christopher S. Bond Life Sciences Center University of Missouri Columbia, MO 65211 Office phone: 573-884-4752 Lab Phone: 573-884-4799 FAX: 573-884-9676 email: staceyg@missouri.edu website: http://psu.missouri.edu/staceylab/index.htm NCSB site: http://www.soybiotechcenter.org NSF Project site: http://www.soybeangenome.org From: Greene, Richard [mailto:Richard.Greene@science.doe.gov] Sent: Wednesday, March 12, 2008 4:38 PM To: undisclosed-recipients Subject: DOE Position The U.S. Department of Energy Office of Science, Office of Basic Energy Sciences is seeking two Energy Biosciences program managers - one for the Photosynthetic Systems Program and one for the Physical Biosciences Program. Richard Greene, who currently manages these programs is also the Lead for the new Photo- and Bio-Chemistry Team in which these programs are housed. Joining the Biosciences Programs in this team is the Solar Photochemistry program. The team is designed to promote cross-disciplinary research that will lay the foundation for breakthroughs in future energy capture and conversion strategies. Job Opportunities: Program Managers for Photosynthetic Systems and Physical Biosciences The Office of Basic Energy Sciences (http://www.sc.doe.gov/bes/bes.html ), Office of Science, US Department of Energy, is seeking qualified applicants for two career federal positions managing either the Photosynthetic Systems Program or the Physical Biosciences Program. Both programs fund mission-oriented, basic science on plant and non-medical microbial systems at universities and national laboratories. The Photosynthetic Systems Program supports fundamental research on the biological conversion of solar energy into chemically stored forms of energy. This entails studies on light harvesting, exciton transfer, charge separation, transfer of reductant to carbon dioxide, as well as the biochemistry of carbon fixation and carbon storage. Areas where biological sciences intersect heavily with energy-relevant chemical sciences and physics, such as in self-assembly of nanoscale components, efficient photon capture and charge separation, predictive design of catalysts, and self-repairing systems, are accentuated. The Physical Biosciences Program combines experimental and computational tools from the physical sciences with biochemistry and molecular biology. The interdisciplinary approach provides a fundamental understanding of the complex processes that convert and store energy in living systems. Research supported includes studies that investigate the mechanisms by which energy transduction systems are assembled and maintained, the processes that regulate energy-relevant chemical reactions within the cell, the underlying biochemical and biophysical principals that determine the architecture of biopolymers and the plant cell wall, and active site protein chemistry that provides a basis for highly selective and efficient bioinspired catalysts. Announcements and on-line application instructions can be found via the BES website: http://www.sc.doe.gov/bes/BESjobs.html or directly at USA Jobs: To apply as a chemist use the following url: http://jobsearch.usajobs.gov/ftva.asp?seeker=1&JobID=69368527 . To apply as a biologist use the following url: http://jobsearch.usajobs.gov/ftva.asp?seeker=1&JobID=69368677 . Applications must be submitted on or before May 9, 2008. The complementary Photosynthetic System and Physical Biosciences Programs are described at: http://www.sc.doe.gov/bes/eb/ebhome.html . These programs also intersect with other research programs of the BES Division of Chemical Sciences, Geosciences, and Biosciences Division, described at http://www.sc.doe.gov/bes/chm/chmhome.html . For questions about this position and working at BES, please contact Richard Greene, richard.greene@science.doe.gov or Eric Rohlfing, eric.rohlfing@science.doe.gov . From jdfriesner from ucdavis.edu Wed Mar 12 17:17:34 2008 From: jdfriesner from ucdavis.edu (Joanna Friesner) Date: Wed Mar 12 17:45:54 2008 Subject: [Arabidopsis] 2008 Arabidopsis meeting: Funding, Registration, Workshops, T-shirts Message-ID: <001101c8848e$dffb25e0$5318eda9@mcb.ucdavis.edu> *Second call for the 2008 Conference* Note that a list of registered attendees is now posted at the conference website. Registration for the 19th International Conference on Arabidopsis Research is now open at: http://www.plantconferences.org/Arabidopsis2008/ -Reduced Fee Deadline: April 30, 2008 (special lower fee for students and postdocs) -Conference Funding: ALL applications are due by APRIL 4, 2008 (1) Plant Biologists in the US- accepting applications for Registration Fee Awards AND for full funding of under-represented minorities (2) Plant Biologists in Canada- accepting applications for Registration Fee Awards (3) Plant Biologists outside of the US and Canada- accepting applications for Registration Fee Awards (Full information is available at the conference website under the 'Funding' link.) -Workshops: Proposals are due by MAY 19, 2008 6 slots for community-proposed and organized workshops are available. A brief application is required and the conference committee will select and approve up to 6 workshop proposals. (Full information is available at the conference website under the 'Workshops' link.) -Abstract submission for ORAL consideration (for Concurrent Sessions) MUST BE submitted by April 30, 2008. After that date, all submissions will be for POSTERS only. (Full information on the website under the 'Abstracts' link.) -Housing Reservation is open online- McGill University's newest residence hall (converted from a hotel) is reserved for graduate student and postdoc housing. Regular attendees can reserve rooms at the Hyatt Regency, the conference site. (Full information on the website under the 'Housing' link.) Rooms are 'first come, first served.' -Conference t-shirt: The design is featured on the registration page and shirts can be pre-ordered during registration. Shirts will also be sold at the conference but quantities may be limited so order during registration to guarantee the color and size you want. We look forward to seeing you all in Montreal this July! Joanna Friesner ICAR 2008 Coordinator On behalf of the NAASC and Conference Organizing Committee 19th International Conference on Arabidopsis Research July 23-37, 2008 Montreal, Canada http://www.plantconferences.org/Arabidopsis2008/ From raj from Ag.arizona.edu Wed Mar 12 18:19:10 2008 From: raj from Ag.arizona.edu (Rich Jorgensen) Date: Thu Mar 13 15:17:29 2008 Subject: [Arabidopsis] The iPlant Collaborative: what potential does offer for Arabidopsis research? Message-ID: <7157E12E-30F4-4B0A-AB4A-7801D961CC00@Ag.arizona.edu> Dear Arabidopsis colleagues, I'm writing to encourage you and your colleagues invest a little time in understanding the iPlant Collaborative and thinking about what it might do for the plant sciences. Background information can be found at www.iplantcollaborative.org, including the NSF solicitation, our proposal, site visit questions and answers, a ppt presentation and other documents, as well as 1-2 page backgrounders on different aspects of the project. I would also suggest that you consider participating in the iPlant Collaborative's April kickoff conference at Cold Spring Harbor Lab, either in person or via our free, live webcast which will allow for direct participation (details at www.iplantcollaborative.org ). Participation in the conference is NOT necessary for participation in the Collaborative, but may be helpful in understanding how best to participate. The first principle of the iPlant Collaborative (our "prime directive", one might say) is that it must be "by, for and of the community". A second major principle is that the iPC's cyberinfrastructure designs must be driven by specific, compelling, and tractable Grand Challenges in the plant sciences. A third major principle is that the Collaborative must serve the entire breadth of the plant sciences, including ecology, evolution and organismic biology as much as the molecular, cellular and developmental disciplines, preferably through Grand Challenges integrated across levels, from the molecular to the organismic to ecosystems. In order to ensure Collaborative resources are dedicated to the most compelling Grand Challenges in the Plant Sciences, the best and the brightest in plant biology will need to invest time and provide leadership to ensure the field assembles and submits the best possible GC proposals to iPlant's external Board of Directors. Importantly, the project is NOT "if we build it, they will come." Rather, the community must first come together and decide WHAT we should build! Until that happens, no cyberinfrastructure will be built. So, the first challenge we face is to engage the community and convince those of you who think deeply about the important questions in the field, as well as comprehend the real down and dirty details of data quality, availability and analysis, to identify the most compelling and tractable Grand Challenges that require computational approaches and cyberinfrastructure development. (see iPlant's community wiki to contribute to discussion of what these GC's should be.) Self-forming Grand Challenge Teams are the most direct way to participate in the iPlant Collaborative. Any group can start a Grand Challenge Team, or propose a Grand Challenge Workshop at which to develop one. GC Teams are central to the iPlant Collaborative because the community through its Board of Directors will choose which Grand Challenges should be prioritized for cyberinfrastructure design and development. Once GC Teams are chosen (our target is 2-4 GCT's before late 2008/early 2009), the iPC's Integrated Solutions Team, led by Lincoln Stein (CSHL) and Sudha Ram (UA), will work with each GCT to design a 'Discovery Environment' to address a particular grand challenge. Successful development of these prototype cyberinfrastructures (Discovery Environments) will require close interaction between IS Team and GC Team members. (See the Grand Challenge Process tab at our web portal for more details.) To ensure community buy-in and ownership of the Collaborative, an independent Board of Directors has been selected which will set priorities for the allocation of Collaborative resources to particular grand challenges, through a process involving self-forming grand challenge teams that will arise from the community and make proposals to the Board. The PI's will be available to facilitate the efforts of GC teams, but we are agnostic about which grand challenges should be prioritized. So, rather than "build it and they will come" the approach of iPC is to get to the community to come and then build what they actually want rather than what we think they might want. To ensure substantial independence, the Board of Directors was appointed through a bootstrapping process, via a Nominating Committee, not by the PI's. One third of the Board will refresh annually, allowing new members of the community to serve. The composition of both the Board of Directors and the Nominating Committee can be found at the project's web portal, www.iplantcollaborative.org . The inaugural Board includes biologists Rob Last (chair), Sabeeha Merchant, Jim Birchler, Toby Kellogg, Susan Singer, Russ Monson, David Rand, Jean-Philippe Vielle and several others to be recruited, mainly in the EEOB area and internationally so that field will be well represented. An equal number of Board members represents the computing research community, from bioinformatics to computational biology to computer science, information science, and computing infrastructure, in order to be able to determine which proposals are really tractable and to guide Collaborative staff in designing the right CI. Thus, the Board will possess diverse, balanced expertise with which to evaluate any Grand Challenge proposal submitted by the community. Self-forming Grand Challenge Teams do not need to wait for the conference in April to get started. The conference is an opportunity for plant and computing researchers to get together and so attendance is one way to foster or participate in formation of GC Teams. It is not obligatory for participation in the project (though we do hope to have broad representation of the full range of plant biologists and computing researchers so that discussions will be high quality and balanced). The conference is NOT a bioinformatics meeting - it is a biology conference aimed at understanding which are the most compelling and tractable grand challenges in the plant sciences that will benefit from cyberinfrastructure development. The conference will be webcast live, allowing for direct participation in discussions over the web (and will be archived for later viewing). You can participate on your laptop. Another suggestion I would offer would be for interested campuses to arrange a common webcast location (requiring only a computer, web access and a projector) where campus researchers could come together to participate in and discuss the conference - we will have facilitators to ensure all persons can participate in discussion. Some institutions are also holding pre-meetings to discuss the project: what it might mean for the campus and how to participate most effectively in the Grand Challenge identification process which will define the direction of the project. I hope the Arabidopsis community will consider participating substantively so it will not be left out of the conversation, and so it will be positioned to participate prominently in the Collaborative as it develops. (Program and pre- registration links for both in-person and virtual attendance are at the project's web portal.) The iPlant Collaborative is funded by NSF's Plant Sciences Cyberinfrastructure Collaborative program as a $50M grant over 5 years to develop a cyberinfrastructure for the plant sciences, from molecules and cells to organisms, ecosystems and evolution. As plant biologists, we are all fortunate to have this opportunity to lead biology cyberinfrastructure development. The plant biology community has been entrusted with the opportunity and responsibility because, I believe, we as a field have shown exceptional openness, creativity and leadership across disciplines and experimental organisms. What better community than plant scientists could NSF chosen for this program? Also, had it not been the plant sciences, these funds would presumably have gone instead to other areas of biology, not to plant biology. So, this is an extraordinary opportunity for all plant scientist, and one that we can all feel proud to have obtained. Feel free to pass this information along to your colleagues. I look forward to seeing you at CSHL, either online or in person, for what I believe promises to be a pivotal event for plant biology. I am able to waive onsite costs to increase diversity in the conference so please don't hesitate to ask if you feel you are in that category (flexibly defined). I am available any time to discuss any aspect of the project. Best regards to all, Rich Jorgensen Richard A. Jorgensen Plant Sciences & BIO5 , University of Arizona Director, The iPlant Collaborative www.iplantcollaborative.org From sgrant from email.unc.edu Thu Mar 13 10:23:49 2008 From: sgrant from email.unc.edu (Sarah Grant) Date: Thu Mar 13 15:17:37 2008 Subject: [Arabidopsis] Ws-0 2-hybrid library Message-ID: <003301c8851e$3f4bbb60$6701a8c0@bio.unc.edu> We are looking for a yeast two hybrid library made from leaves or seedlings of the Ws-0 ecotype. We would appreciate any information sent to the following email address sgrant@email.unc.edu Sarah R. Grant Research Associate Professor Department of Biology CB# 3280 University of North Carolina at Chapel Hill Chapel Hill NC 27599 Tel: 919 962-0684 Fax: 919 962-1625 email: sgrant@email.unc.edu Web page: www.bio.unc.edu/faculty/grant/ From jay62728 from yahoo.com Thu Mar 13 16:21:43 2008 From: jay62728 from yahoo.com (Jayanta Chatterjee) Date: Fri Mar 14 17:55:54 2008 Subject: [Arabidopsis] Info regarding binary vectors pGSA1276 and pGSA1165 Message-ID: <659380.18071.qm@web32704.mail.mud.yahoo.com> Hi, Has anyone used the following binary vectors: pGSA1276 and pGSA1165 (from ABRC)? I like to know his/her experience regarding those, particularly regarding kanamycin resistanec as a selection marker in plants (Arabidopsis)? Thank you. Regards, Jayanta --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From yuanqing from ualberta.ca Fri Mar 14 10:48:54 2008 From: yuanqing from ualberta.ca (yuanqing@ualberta.ca) Date: Fri Mar 14 17:56:01 2008 Subject: [Arabidopsis] JA and ABA treatments Message-ID: <20080314094854.2yp45ofyoco084o0@webmail.ualberta.ca> Hi, colleagues, I am going to treat plants with JA and ABA for Northern blotting analysis. Should I cover the plants after spraying 50 uM of JA and ABA separately? I have those high plastic colorless covers that can cover the whole trays. I do not have separate chambers for treated plants and mock plants; I have to place them in the same chamber. I will try to separate them by some distance. Someone in the lab said I do not need to cover them, while I think that they should be covered to avoid influence each other as they are in the same chamber. Any reply would be appreciated. Thanks. From frist from cc.umanitoba.ca Sat Mar 15 13:59:17 2008 From: frist from cc.umanitoba.ca (Brian Fristensky) Date: Sun Mar 16 00:43:38 2008 Subject: [Arabidopsis] Bioinformatics course - Mississauga, Ontario, Canada Message-ID: <1205607557.29903.4.camel@merak.cc.umanitoba.ca> --------------------------------------------------- Genome Canada APPLIED COMPUTATIONAL GENOMICS COURSE University of Toronto, Mississauga, Ontario, Canada May 2 - 8, 2008 --------------------------------------------------- The ACGC introduces biologists to some of the latest methods in bioinformatics, including DNA and protein sequence analysis, automation of data analysis, web services and workflows, and high-throughput genome annotation. The course is led by researchers from the Genome Canada Bioinformatics Platform. Both lectures and extensive hands-on tutorial sessions are included. For full details see: For more information visit the website: http://www.gcbioinformatics.ca/training ________________________________________________________________________________ The project is supported by Genome Alberta and Genome Canada, a not-for-profit organization which is leading Canada's national strategy on genomics with $ 600 million in funding from the federal government. From oneusch from essex.ac.uk Sun Mar 16 01:58:19 2008 From: oneusch from essex.ac.uk (Neuschaefer-Rube, Olaf) Date: Sun Mar 16 13:23:21 2008 Subject: [Arabidopsis] Enkeim-2 seeds needed! Message-ID: Hello, I ordered Enkheim-2 (En-2) wildtype seed stock from NASC but, unfortunately, they don't germinate! Is there somebody around who has some seeds of this line for me since I urgently need them as a control for an experiment? Your help is very much appreciated!!! Olaf Neuschaefer-Rube Biol.Sciences, University of Essex, Colchester, UK From loudet from versailles.inra.fr Sun Mar 16 13:55:16 2008 From: loudet from versailles.inra.fr (Olivier Loudet) Date: Mon Mar 17 11:15:57 2008 Subject: [Arabidopsis] Enkeim-2 seeds needed! In-Reply-To: References: Message-ID: <6.2.1.2.2.20080316195130.02e29550@versailles.inra.fr> Try INRA Versailles' Biological Resource Center for natural variation material (accessions, RILs, NILs): Description of material(s): http://dbsgap.versailles.inra.fr/vnat/ Ordering: http://dbsgap.versailles.inra.fr/portail/ [ En-2 = N1138 = 468AV ] Olivier Loudet Le 16/03/2008 07:58,Neuschaefer-Rube, Olaf ?crit: >Hello, >I ordered Enkheim-2 (En-2) wildtype seed stock from NASC but, >unfortunately, they don't germinate! >Is there somebody around who has some seeds of this line for me since I >urgently need them as a control for an experiment? >Your help is very much appreciated!!! >Olaf Neuschaefer-Rube >Biol.Sciences, University of Essex, Colchester, UK O. ________________________________________________________ Olivier Loudet INRA, National Institute for Agronomical Research Versailles, France V.A.S.T group -- Variation and Abiotic Stress Tolerance Mail: loudet@versailles.inra.fr Science: www.inra.fr/vast MyWeb: www.olivier.loudet.net MyOwnGenes: www.teva.loudet.net " Catch your Fire in your Turn and Let it Burn " ________________________________________________________ From gilles.vachon from ujf-grenoble.fr Tue Mar 18 07:32:21 2008 From: gilles.vachon from ujf-grenoble.fr (gilles vachon) Date: Tue Mar 18 23:37:00 2008 Subject: [Arabidopsis] pGADT7-Rec AB vector not available anymore... Message-ID: <003201c888f4$1d0e3fd0$e70d4d98@erable> Hi all, Clontech told us they do not sold nor distribute the one-hybrid/two hybrid vector pGADT7-Rec AB. We?d like to use it to clone into the Sfi sites. Can someone here send us an aliquot of this vector, even with a cloned insert as long as we can remove it with Sfi I ?? Gilles Vachon Laboratoire Plastes et Diff?renciation Cellulaire, FRE 3017, B?t. CERMO, 3eme etage Universite J. Fourier, BP 53X 38041 GRENOBLE CEDEX FRANCE Tel: (33) (0)4 76 51 48 92 Fax: (33) (0)4 76 51 43 36 e-mail: Gilles.Vachon@ujf-grenoble.fr WEB: http://www.ujf-grenoble.fr/PDC/ From james.stark from umb.edu Tue Mar 18 16:12:25 2008 From: james.stark from umb.edu (stark) Date: Tue Mar 18 23:37:10 2008 Subject: [Arabidopsis] seeds for lateral root inititation marker line LRB10 Message-ID: <47E03039.4020602@umb.edu> I'm looking for seeds of LRB10, an enhancer trap GUS line that marks the earliest stage of Arabidopsis lateral root initiation, as described in Malamy and Benfey 1997, Development 124, 33-44. The Benfey lab apparently no longer has viable seed for this line, and I'm hoping that perhaps someone else has used it more recently and would still have viable seed. Thanks for any leads. Jim Stark UMass Boston From pablojenik from aya.yale.edu Wed Mar 19 09:07:58 2008 From: pablojenik from aya.yale.edu (Pablo JENIK) Date: Wed Mar 19 18:38:37 2008 Subject: [Arabidopsis] Reporters for chloroplast Message-ID: <446fce520803190707m6d1e4002h20b52713f60ede78@mail.gmail.com> I'm characterizing a mutant that seems to have defects in chloroplast development. I was wondering whether somebody can provide or point to chloroplast reporter genes, this is nuclear genes with products targeted to the chloroplast or otherwise related to chloroplast development. In particular if any are known that would illuminate different stages in chloroplast development. Transcriptional or translational fusions, GUS or GFP, all are welcome. Thank you in advance for your help. Pablo -- Pablo Jenik, Ph.D. Visiting Assistant Professor Department of Biology Franklin and Marshall College P.O. Box 3003 Lancaster, PA 17604-3003 (717) 358-4431 From nicholas.provart from utoronto.ca Wed Mar 19 16:40:32 2008 From: nicholas.provart from utoronto.ca (Nicholas Provart) Date: Wed Mar 19 18:38:43 2008 Subject: [Arabidopsis] 6th Canadian Plant Genomics Conference: June 23rd-26th Toronto, Canada. Message-ID: <009a01c88a09$db82cba0$5bd6968e@uic00o3tkmejay> Hello everyone, The 6th Canadian Plant Genomics Workshop will bring together Canadian researchers and internationally-renowned scientists to explore recent advances in genomics research and how these advances are being integrated within the plant sciences. The workshop will examine the latest developments in genomics; insights into the processes and pathways that control plant development, metabolism, or interactions with the environment and other organisms; and new opportunities for biotechnology/biofuels. The workshop will be held June 23rd - 26th, 2008 in Toronto. Critical dates February 1st: registration opens April 11th: early bird registration deadline April 11th: deadline for abstracts to be considered for oral presentation May 1st: deadline for abstracts to be included in abstract book May 24th: accommodation registration deadline For more information, including a list of keynote speakers, and online registration and abstract submission instructions, see the conference website at http://cpgw2008.cagef.utoronto.ca/. Best regards, Nick ......................................... Nicholas Provart, PhD Assistant Professor, Plant Bioinformatics & Systems Biology Director, Graduate Program in Genome Biology and Bioinformatics Member, Centre for the Analysis of Genome Evolution and Function Rm 3051, Dept. of Cell and Systems Biology, Uni. Toronto 25 Willcocks St., Toronto, ON. M5S 3B2. CANADA Tel. (+1) (416) 978-7141, Fax. (+1) (416) 978-5878 URL. http://www.csb.utoronto.ca/faculty/provart/ Arabidopsis Tools. http://www.bar.utoronto.ca email. nicholas.provart@utoronto.ca TTC. Spadina LRT, Willcocks St. From eblancaflor from noble.org Sun Mar 23 19:57:39 2008 From: eblancaflor from noble.org (Blancaflor, Elison) Date: Mon Mar 24 00:59:42 2008 Subject: [Arabidopsis] RE: Postdoctoral position in plant lipid signaling In-Reply-To: 200803201703.m2KH3cL00288@net.bio.net Message-ID: <497D588A106806408FB0792E4E9A1658016F7841@mail2.noble.org> Postdoctoral Fellow Position number: PB-S095-440 The Samuel Roberts Noble Foundation is seeking a postdoctoral fellow in the laboratory of Dr. Elison Blancaflor beginning in June, 2008 to work on characterizing the role of the N-acylethanolamine (NAE) group of lipid mediators in plant development and response to environmental stimuli. Studies will focus on Arabidopsis mutants in genes that encode fatty acid amide hydrolases (FAAH), the enzymes that breakdown NAEs. This project which is funded by the Department of Energy (DOE) Biosciences program will be conducted in collaboration with the group of Dr. Kent Chapman at the University of North Texas. This project is funded annually with extensions up to a total of three years contingent on performance and continued funding by the DOE. For more information please see our publications related to this project Blancaflor et al. (2003) Planta 217: 206-217; Wang et al (2006) PNAS 103: 12197-12202; Teaster et al. (2007) Plant Cell 19:2454-2469. A Ph.D. in Plant Biology or Cell/Molecular Biology is required. The Samuel Roberts Noble Foundation has state-of-the-art research, greenhouse facilities, modern laboratory and office areas and excellent benefits. Application Instructions: Applicants are requested to apply online by completing the application and submitting a resume, copy (s) of college transcripts, contact information for three references (excluding relatives), and a cover letter explaining interest in the position and career goals. If applicant does not have the ability to upload the additional documents they can be faxed to (580) 224-6240 (please include position number), but the application should be completed and submitted online. Applications will be accepted until a candidate is hired. Interested applicants should apply immediately. The Samuel Roberts Noble Foundation, Inc. Human Resources Department Position Number: PB-S095-440 P.O. Box 2180 Ardmore, OK 73402 Website: http://www.noble.org/ The Samuel Roberts Noble Foundation, Inc., is an Equal Opportunity Employer and will consider all qualified applicants for employment, regardless of their race, color, creed, gender, national origin, age, disability, or veteran status. From rgutierrez from bio.puc.cl Mon Mar 24 16:04:13 2008 From: rgutierrez from bio.puc.cl (Rodrigo A. Gutierrez) Date: Mon Mar 24 16:28:02 2008 Subject: [Arabidopsis] Help for outreach project - Plant Science and Biotechnology Education Program Message-ID: <009701c88df2$9d470b20$d7d52160$@puc.cl> Dear friends and colleagues, We are currently leading a science education project in Chile. Our goal is to promote a better understanding and appreciation of plants and their biotechnological uses. Our program is oriented towards the entire community, with a special focus on high school students. For this project we need Arabidopsis lines with dramatic visual phenotypes in response to pathogen infection, high salt, freezing, drought, heavy metals, or any other biotic/abiotic stress treatment that can produce clear visual phenotypes. We have searched the ABRC stocks but have found a very limited number of lines meeting this criteria. Also, we would greatly appreciate direct input from the experts to maximize the phenotypes in each line. The goal of our outreach project is to develop a permanent exhibition at The Science and Technology Museum in Santiago, Chile. One of the sections we plan on developing is an exhibition showing Arabidopsis genotypes grown under different environmental conditions. This section has several purposes: 1) Introduction of Arabidopsis and the importance of a model system for research. 2) Introduction of concepts such as phenotypes and their alteration by genetic modification. 3) Creation of a "research computer game" on-line using these plants. We hope this game will introduce children to scientific research and biotechnology even in remote areas (with access to internet). We will acknowledge in our exhibit everybody's help by means of their names, institutions and granting agency that funded the production of the material. We ask for seeds that are tolerant or resistant to environmental conditions, and with phenotypes that can be easily visualized under the appropriate conditions. These plants will be used solely for the stated educational purposes, and will not be used for research or for biotechnological applications. We would be happy to provide additional details about our outreach project if necessary. Please send your seeds with instructions for growth and stress treatments to: Dr. Rodrigo A. Gutierrez Profesor Asistente Departamento de Gen?tica Molecular y Microbiolog?a. Facultad de Ciencias Biol?gicas Pontificia Universidad Catolica de Chile. Avda. Libertador Bernardo O'Higgins 340. Santiago. 8331010. Chile Thank you very much in advance for your support. Dr. Rodrigo A. Gutierrez Assistant Professor Department of Molecular Genetics & Microbiology P. Universidad Catolica de Chile. Alameda 340. Santiago. 8331010. Chile P: (56-2) 686-2663 | F: (56-2) 222-5515 rgutierrez@bio.puc.cl From cxde from iastate.edu Tue Mar 25 13:49:35 2008 From: cxde from iastate.edu (Xi Chen) Date: Wed Mar 26 19:05:35 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds Message-ID: <8af797d40803251149j59a0a1dcvabd04d64917a4b6f@mail.gmail.com> Hello the members of this e-mail group, When I grew the Arabidopsis, the plants were affected by thrip. Now I guess the seed may have the eggs of thrip. So do you guys have any good idea for killing the eggs of thrip on Arabidopsis seed? Thanks so much! Best, Xi From mprigge from indiana.edu Wed Mar 26 19:44:47 2008 From: mprigge from indiana.edu (Michael J. Prigge) Date: Wed Mar 26 23:48:51 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds In-Reply-To: <8af797d40803251149j59a0a1dcvabd04d64917a4b6f@mail.gmail.com> References: <8af797d40803251149j59a0a1dcvabd04d64917a4b6f@mail.gmail.com> Message-ID: <8058B70A-9AFA-4529-BB01-035DF470A0D9@indiana.edu> I have heard that freezing the (dry) seed in the -80?C freezer overnight can kill the eggs without affecting seed viability too much. (I haven't tried it myself.) Good Luck, Mike On Mar 25, 2008, at 2:49 PM, Xi Chen wrote: > Hello the members of this e-mail group, > > When I grew the Arabidopsis, the plants were affected by thrip. Now > I guess > the seed may have the eggs of thrip. So do you guys have any good > idea for > killing the eggs of thrip on Arabidopsis seed? Thanks so much! > > Best, > > Xi From j.mylne from imb.uq.edu.au Wed Mar 26 20:02:56 2008 From: j.mylne from imb.uq.edu.au (Josh Mylne) Date: Wed Mar 26 23:48:58 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds In-Reply-To: <8af797d40803251149j59a0a1dcvabd04d64917a4b6f@mail.gmail.com> References: <8af797d40803251149j59a0a1dcvabd04d64917a4b6f@mail.gmail.com> Message-ID: <003701c88fa6$4ab38040$93756682@IMBPC.AD> Hi Xi, The thrips can actually lays eggs that end up INSIDE the seeds. I have sown sterilized seeds from infested plants on plates and the thrips came out of the seeds when they germinated. To clear the infestation, in combination with spraying the room I'd recommend sowing on plates and only pricking plants that have no thrips. Josh ---- Office: +61 7 3346 2021 E-mail: j.mylne@imb.uq.edu.au -----Original Message----- From: arab-gen-bounces@oat.bio.indiana.edu [mailto:arab-gen-bounces@oat.bio.indiana.edu] On Behalf Of Xi Chen Sent: Wednesday, 26 March 2008 4:50 AM To: arab-gen@magpie.bio.indiana.edu Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds Hello the members of this e-mail group, When I grew the Arabidopsis, the plants were affected by thrip. Now I guess the seed may have the eggs of thrip. So do you guys have any good idea for killing the eggs of thrip on Arabidopsis seed? Thanks so much! Best, Xi _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen From DLoque from lbl.gov Thu Mar 27 01:52:28 2008 From: DLoque from lbl.gov (Dominique Loque) Date: Thu Mar 27 11:50:06 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds In-Reply-To: <8058B70A-9AFA-4529-BB01-035DF470A0D9@indiana.edu> References: <8af797d40803251149j59a0a1dcvabd04d64917a4b6f@mail.gmail.com> <8058B70A-9AFA-4529-BB01-035DF470A0D9@indiana.edu> Message-ID: Hi I have used -80?C for overnight many time and seems working pretty well. The seed viability was not affected. I have been told that 2h was enough, but I have never tried. Good Luck Dominique On Mar 26, 2008, at 5:44 PM, Michael J. Prigge wrote: > I have heard that freezing the (dry) seed in the -80?C freezer > overnight can kill the eggs without affecting seed viability too > much. (I haven't tried it myself.) > Good Luck, > Mike > > On Mar 25, 2008, at 2:49 PM, Xi Chen wrote: > >> Hello the members of this e-mail group, >> >> When I grew the Arabidopsis, the plants were affected by thrip. Now >> I guess >> the seed may have the eggs of thrip. So do you guys have any good >> idea for >> killing the eggs of thrip on Arabidopsis seed? Thanks so much! >> >> Best, >> >> Xi > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen Dominique Loque Mailing Address: LBNL 1 Cyclotron Road, MS 977R152 Berkeley, CA 94720-8205 Physical Address: JBEI/LBNL Bldg 977 717 Potter St. Berkeley, CA 94710-2722 phone: (510) 486-7332 or (510) 207 0372 fax: (510) 486-4252 Email: dloque@lbl.gov From sbsung from mail.utexas.edu Thu Mar 27 08:57:48 2008 From: sbsung from mail.utexas.edu (Sibum Sung) Date: Thu Mar 27 11:50:12 2008 Subject: [Arabidopsis] Postdoctoral position at UT-Austin Message-ID: <001001c89012$8ab94d70$a02be850$@utexas.edu> Postdoctoral position at the University of Texas at Austin A postdoctoral position is available to study the molecular mechanism of floral transition. The research mainly focuses on the epigenetic regulation of the floral transition by environmental cues, using genetic, biochemical and epigenomic approaches. Applicants should have a recent Ph.D. in biological sciences or a related discipline. Experience with plants is helpful, but not required. Salary and benefits will be competitive and commensurate with experience. Interested applicants should send a CV, brief summary of research experience, and contact information for at least two referees by email to Sibum Sung at (sbsung@mail.utexas.edu). Sibum Sung Assistant Professor Section of Molecular Cell & Developmental Biology School of Biological Sciences Institute for Cell and Molecular Biology The University of Texas at Austin 1 University Station A6700 Austin, TX 78712-0183 From phmasson from wisc.edu Thu Mar 27 14:44:46 2008 From: phmasson from wisc.edu (Patrick H Masson) Date: Thu Mar 27 16:39:56 2008 Subject: [Arabidopsis] Postdoc positions available at the DOE Great Lakes Bioenergy Research Center Message-ID: The new DOE Great Lakes Bioenergy Research Center (GLBRC) has three openings for postdoctoral research on the identification and characterization of Arabidopsis genes and proteins involved in secondary cell wall biosynthesis and deposition. The positions begin as soon as April 2008 and are located at the University of Wisconsin- Madison. Candidates should have a Ph.D. and a strong record of publication in peer-reviewed journals. Position 1 will be located in the Department of Biochemistry. Candidates for this position should have experience with plant tissue culture manipulation, subcellular fractionation, protein analysis and mass spectroscopy. Positions 2 and 3 will be located in the Departments of Botany and Genetics. Candidates for these two positions should have experience with bioinformatics, molecular genetics, and plant cell imaging (microscopy). Applications should be submitted as single pdf files to humanresources@greatlakesbioenergy.org. They should specify the position that they seek, and include a statement of interest, vitae, names and contact information for 3 professional references. For further information, please contact Drs. Sebastian Bednarek, Marisa Otegui, Patrick H. Masson or John Sedbrook. Application review will begin by March 28, 2008. The University of Wisconsin-Madison is an Affirmative Action / Equal Opportunity Employer. From Brian.Kwan from arborgen.co.nz Thu Mar 27 16:02:56 2008 From: Brian.Kwan from arborgen.co.nz (Brian Kwan) Date: Thu Mar 27 16:40:02 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds Message-ID: <46E68C2FBDE21E4E977E1AE78D218F382E28D9@treetech-srv1.treetech.co.nz> You could put the seeds in a box with a pest strip. These are available from supermarkets for hanging in cupboards to kill cockroaches, silverfish etc. The active ingredient is often dichlorovos. Also effective in growth chambers for killing aphids. Regards Brian -----Original Message----- From: arab-gen-bounces@oat.bio.indiana.edu [mailto:arab-gen-bounces@oat.bio.indiana.edu] On Behalf Of Xi Chen Sent: Wednesday, 26 March 2008 4:50 AM To: arab-gen@magpie.bio.indiana.edu Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds Hello the members of this e-mail group, When I grew the Arabidopsis, the plants were affected by thrip. Now I guess the seed may have the eggs of thrip. So do you guys have any good idea for killing the eggs of thrip on Arabidopsis seed? Thanks so much! Best, Xi _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen From Thomas.Girin from bbsrc.ac.uk Thu Mar 27 19:02:51 2008 From: Thomas.Girin from bbsrc.ac.uk (Thomas Girin (JIC)) Date: Fri Mar 28 12:05:01 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds References: <46E68C2FBDE21E4E977E1AE78D218F382E28D9@treetech-srv1.treetech.co.nz> Message-ID: In our institut we leave the seeds (in 2 bag layers) in the freezer over night, it is supposed to kill all the eggs. Regards, Thomas. -----Original Message----- From: arab-gen-bounces@oat.bio.indiana.edu on behalf of Brian Kwan Sent: Thu 27/03/2008 21:02 To: arab-gen@magpie.bio.indiana.edu Subject: RE: [Arabidopsis] How to kill the thrip on Arabidopsis seeds You could put the seeds in a box with a pest strip. These are available from supermarkets for hanging in cupboards to kill cockroaches, silverfish etc. The active ingredient is often dichlorovos. Also effective in growth chambers for killing aphids. Regards Brian -----Original Message----- From: arab-gen-bounces@oat.bio.indiana.edu [mailto:arab-gen-bounces@oat.bio.indiana.edu] On Behalf Of Xi Chen Sent: Wednesday, 26 March 2008 4:50 AM To: arab-gen@magpie.bio.indiana.edu Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds Hello the members of this e-mail group, When I grew the Arabidopsis, the plants were affected by thrip. Now I guess the seed may have the eggs of thrip. So do you guys have any good idea for killing the eggs of thrip on Arabidopsis seed? Thanks so much! Best, Xi _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen _______________________________________________ Arab-gen mailing list Arab-gen@net.bio.net http://www.bio.net/biomail/listinfo/arab-gen From cohen047 from umn.edu Fri Mar 28 14:46:51 2008 From: cohen047 from umn.edu (Jerry D. Cohen) Date: Sat Mar 29 01:06:42 2008 Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds In-Reply-To: References: <46E68C2FBDE21E4E977E1AE78D218F382E28D9@treetech-srv1.treetech.co.nz> Message-ID: <47ED4B2B.4070705@umn.edu> I have not tried it with Arabidopsis (only because I never had this problem), but a very clean way to deal with this is to put the seeds under a high level of CO2 (from a few pellets of dry ice in a plastic bag). Seeds are much more tolerant of elevated CO2 than eggs and other forms of live insects. Usually 2-3 days of such treatment works well, but we have had no problems with much longer times with many kinds of seeds. Cheers, Jerry Thomas Girin (JIC) wrote: > In our institut we leave the seeds (in 2 bag layers) in the freezer over night, it is supposed to kill all the eggs. > Regards, > Thomas. > > -----Original Message----- > From: arab-gen-bounces@oat.bio.indiana.edu on behalf of Brian Kwan > Sent: Thu 27/03/2008 21:02 > To: arab-gen@magpie.bio.indiana.edu > Subject: RE: [Arabidopsis] How to kill the thrip on Arabidopsis seeds > > > You could put the seeds in a box with a pest strip. These are available from supermarkets for hanging in cupboards to kill cockroaches, silverfish etc. The active ingredient is often dichlorovos. Also effective in growth chambers for killing aphids. > > Regards Brian > > > -----Original Message----- > From: arab-gen-bounces@oat.bio.indiana.edu > [mailto:arab-gen-bounces@oat.bio.indiana.edu] On Behalf Of Xi Chen > Sent: Wednesday, 26 March 2008 4:50 AM > To: arab-gen@magpie.bio.indiana.edu > Subject: [Arabidopsis] How to kill the thrip on Arabidopsis seeds > > Hello the members of this e-mail group, > > When I grew the Arabidopsis, the plants were affected by thrip. Now I guess > the seed may have the eggs of thrip. So do you guys have any good idea for > killing the eggs of thrip on Arabidopsis seed? Thanks so much! > > Best, > > Xi > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > -- ?????? From altpeter from ufl.edu Sun Mar 30 15:03:11 2008 From: altpeter from ufl.edu (Altpeter,Fredy) Date: Sun Mar 30 18:47:02 2008 Subject: [Arabidopsis] DOE/USDA funded Postdoctoral Research Associate Position, Ph.D and MS Assistantships and Internships in Bioenergy-Biotechnology of Sugarcane In-Reply-To: <18967E83B2946D4DBB46899C01D36A73117D50A767@UFEXCH-MBXCL01.ad.ufl.edu> References: <18967E83B2946D4DBB46899C01D36A73117D50A767@UFEXCH-MBXCL01.ad.ufl.edu> Message-ID: <18967E83B2946D4DBB46899C01D36A73117D50A768@UFEXCH-MBXCL01.ad.ufl.edu> Please post the following job opening to the arabidopsis mailgroup. Thanks Fredy ********************************************************** Fredy Altpeter Assistant Professor Laboratory of Molecular Plant Physiology Agronomy Department University of Florida, IFAS PO Box 11O300 2191 McCarty Hall Gainesville, FL32611-300 Tel.: 352 392 1823 ext. 204, 216 or 218 Fax: 352 392 7248 E-mail: altpeter@ufl.edu http://agronomy.ifas.ufl.edu/faculty/altpeter.shtml http://pmcb.ifas.ufl.edu/faculty.htm http://www.ufgi.ufl.edu/aspzzz/members1.asp http://www.energy.ufl.edu/index.php?src=faculty&ID=79 ********************************************************** DOE/USDA funded Postdoctoral Research Associate Position, Ph.D and MS Assistantships and Internships in Bioenergy-Biotechnology of Sugarcane DOE-USDA funded (http://www.energy.gov/6035.htm) positions are available for postdoctoral research associate, Ph.D. and MS graduate students and interns. Assistantships are available for highly motivated and qualified students in the Laboratory of Plant Molecular Physiology, Agronomy Department, IFAS, University of Florida. Research can begin immediately and coursework can start in the fall of 2008 or spring of 2009. Lignocellulosic materials produced by biomass grasses are a promising feedstock to produce low-cost ethanol. Our research program integrates advanced genetic transformation technology and functional genomics to identify, isolate and engineer limiting factors for molecular improvement of grasses http://agronomy.ifas.ufl.edu/pdfs/Genes.pdf. Funded research currently focuses on three objectives to develop genetically enhanced sugarcane for bioenergy production (http://agnetonline.com/2008/03/24/uf-receives-1m-to-unlock-energy-from-sugarcane-residues/) Postdoc and thesis research will focus on the genetic improvement of biomass quantity and quality in sugarcane for production of biofuel. This involves gene expression profiling, gene isolation, construction of plant expression vectors, stable genetic transformation of sugarcane (using well established transformation protocols and developing advanced technology) with candidate genes, molecular, physiological and biochemical characterization of transgenic sugarcane, evaluation of biomass conversion to bioethanol. This is an ideal opportunity for meaningful publications and future professional development in either academia or industry. Excellent communication skills and a strong publication record demonstrating expertise in a range of molecular techniques are required for the postdoctoral position to support grant proposal development and lead a team of students. An earned M.S. degree in agronomy, plant breeding, or a related field is required for PhD assistantships. Previous experience with molecular techniques and tissue culture are desirable. A plant science or biotechnology background is necessary for the MS assistantships. Internships are available for junior and senior students in plant or biological sciences. Interested applicants should send: 1) Current resume including names, addresses, and telephone numbers of three references 2) GRE scores and 3) a letter of application including statement of purpose and qualifications. In addition, international students must include a copy of their TOEFL scores. Applications and questions related to the application process should preferably be send by e-mail to faltpeter@ifas.ufl.edu or addressed to: Dr. Fredy Altpeter Agronomy Department University of Florida, IFAS PO Box 11O300 Gainesville, FL 32611-300 Review of applications will start immediately and continue until a suitable candidate is identified. Gainesville, the home town of the University of Florida, is located half way between the Atlantic Ocean and the Gulf of Mexico with a population of 140 000 (46 000 students) and has frequently achieved top ratings in quality of life and affordability http://gainesville.com/apps/pbcs.dll/article?AID=/20070506/SUNFRONT/705060331&SearchID=73280328541268. More information on the University of Florida is available at http://www.ufl.edu University of Florida is an Affirmative Action/Equal Opportunity Employer (male/female) and does not discriminate on the basis of race, color, creed, religion, national origin, ancestry, citizenship, age, gender, sexual orientation, disability, or Vietnam Era Veteran status in the offering of all benefits, services, and educational and employment opportunities. Women and minorities are encouraged to apply.