[Arabidopsis] Asking whole mount in situ hybridization with seedling

Dong-Keun Lee via arab-gen%40net.bio.net (by d-k.lee from utah.edu)
Fri Oct 31 12:57:43 EST 2008


Hi 

I am doing whole mount in situ hybridization with 2 day old seedling.

Every time, I got strong non-specific signals in mature regions of roots,
hypocotyls, and cotyledons. 

I am wondering it is common or there is some tip to reduce the non-specific
signals?

 

Dk Lee



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