[Arabidopsis] problem in pcr

rupesh tayade via arab-gen%40net.bio.net (by rupesh.tayade from gmail.com)
Wed Sep 10 01:43:37 EST 2008


i am working on tomato disease resistant gene screening i.e MAS( marker
assisted selection) . i have did the DNA isolation from
green house plant and open field plant. and complete the pcr for diffent
resistant gene. for near about 1500 sample the pcr is working fine got the
good amplification, but when i have again collected the samples after 30 day
the DNA Quality looks good on gel ,but not get amplified in pcr reaction. we
are tried the things but its not working. i would be thankful if some one
suggest me to over come the problem.

lookinforward for suggestion


With Regards

Rupesh


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