[Arabidopsis] Binary BAC transformation
(by mocliamgtoh from hotmail.com)
Sun Aug 30 18:45:46 EST 2009
I am writing to seek suggestions on transforming Binary BAC (BiBAC or TAC) =
into Arabidopsis. I need to transform a few BiBAC clones into Arabidospis =
C24 and Col ecotypes hopefully by flower dipping. Can anyone share with me=
a detailed protocol for BiBAC transformation? In addition=2C it will be h=
elpful to know the efficiency of BiBAC transformation comparing with routin=
e binary vectors. Are there any special strains preferred instead of regul=
ar GV3101 Agrobacteria?
For BAC handling=2C can tri-parental mating be used to introduce BiBAC into=
Agrobacteria. If so=2C can I have this protocol as well?
Any suggestions or sharing will be greatly appreciated!
Department of Biological Science
California State University=2C Fullerton
Windows Live: Make it easier for your friends to see what you=92re up to on=
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