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[Arabidopsis] RNA extraction from dry seed

yuanqing from ualberta.ca via arab-gen%40net.bio.net (by yuanqing from ualberta.ca)
Tue Feb 3 19:50:59 EST 2009


Most kits do not work with dry seeds, in my view.
I ever extracted total RNA from dry At seeds using the following  
protocol and got RNA comparable to young tissue RNA extracted using  
Qiagen Plant RNeasy mini kit. Good luck!

Suzuki Y, Kawazu T, Koyama H (2004) RNA isolation from siliques,
dry seeds, and other tissues of Arabidopsis thaliana. Biotechniques

Quoting "Zacharie LeBlanc" <zleblanc from uoguelph.ca>:

> Hi all, I'm trying to extract RNA from dry Arabidopsis seeds in order to do
> some expression profiling. I'm currently using a homogenizer in order to
> grind 0.1 grams of seeds then use the protocol given along with Plant RNA
> Reagent by Invitrogen. verification of my product on a 0.1% agarose gel
> hasn't yielded any positive results yet. any thoughts?
> -Zach LeBlanc
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