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[Arabidopsis] RE: RNA extraction from dry seed

Edwin Reidel via arab-gen%40net.bio.net (by edwin.reidel from nunhems.com)
Wed Feb 4 13:33:35 EST 2009

It's hard to beat the modified hot borate buffer developed for RNA 
extraction from cotton seeds by Wan & Wilkins:  Analytical Biochemistry: 
223(1): 7-12.

Also check the citing papers for further modifications significantly 
simplifying their protocol, especially Wu et al. (Plant Molecular Biology 
Reporter.  20(3):  213-218)  I routinely extracted intact, high-purity RNA 
from various plant tissues with the hot borate buffer.

Edwin J. Reidel
Scientist, Seed Production Research
Nunhems USA, Inc.


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