[Arabidopsis] RE: RNA extraction from dry seed
(by edwin.reidel from nunhems.com)
Wed Feb 4 13:33:35 EST 2009
It's hard to beat the modified hot borate buffer developed for RNA
extraction from cotton seeds by Wan & Wilkins: Analytical Biochemistry:
Also check the citing papers for further modifications significantly
simplifying their protocol, especially Wu et al. (Plant Molecular Biology
Reporter. 20(3): 213-218) I routinely extracted intact, high-purity RNA
from various plant tissues with the hot borate buffer.
Edwin J. Reidel
Scientist, Seed Production Research
Nunhems USA, Inc.
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