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[Arabidopsis] Wanted info on electrical drill for homogenisation in 1.5 ml tubes

Holt, Ben F. III via arab-gen%40net.bio.net (by benholt from ou.edu)
Fri Feb 6 15:57:23 EST 2009

Hi Pedro,

We have had good luck with the Heidolph Overhead Stirrers - they have some pretty fancy models, but we find the stripped down RZR1 model works great for what you want to do. It isn't a keyless chuck, but we do have people that use the plastic pestles all the time and it has tons of torque. I personally prefer to use a stainless steel bit that is cut to the interior shape (bit smaller for buffer and sample) of a 1.5ml Eppendorf tube. For proteins, a quick wipe down is enough between samples. For DNA that will be amplified, we clean the pestle with a kimwipe and water and just make sure we wipe if off while the bit spins with plenty of water and a couple of wipes with clean parts of the kimwipe. Works great.


Good luck,

On 2/4/09 3:54 AM, "P. Rocha" <procha_ac from yahoo.co.uk> wrote:

Dear colleagues,

I am seeking recommendations for purchasing an electrical drill (or similar device) that can be used with plastic "disposable" pestles for homogenisation of soft plant material (usually Arabidopsis leaves) in 1.5 ml tubes.

Ideally the machine should:

a. Be light enough to be used by hand or that can be safely held on a lab stand

b. Allow easy and quick replacement of the pestles (keyless chuck perhaps?)

c. Sufficient torque to do the job (and not easily get stuck as it happens with lesser machines)

If you have personal successful experience with such wonderful tool I would be grateful if you could let me know its manufacturer and model.

Thank you,

Pedro Rocha

procha_ac from yahoo.co.uk

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Ben Holt
Assistant Professor
University of Oklahoma
Department of Botany and Microbiology
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