[Arabidopsis] Gateway-adapted 35S vectors for transient expression

James Stark via arab-gen%40net.bio.net (by James.Stark from umb.edu)
Tue Feb 17 01:08:31 EST 2009


I am hoping to find one or more Gateway-adapted 35S vectors to use as effector plasmids in protoplast transient expression assays (PEG transfection).  There are many vectors available for Agrobacterium-mediated transformation, but since most are fairly large, smaller plasmids lacking plant selection markers and T-DNA left and right borders would be preferable.  Does anyone know of a such a plain 35S-Gateway cassette vector, or perhaps even versions that add epitope tags to the genes inserted in the Gateway sites?  Any information or leads would be very much appreciated.

Thanks,
Jim Stark
UMass Boston
USA






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