[Arabidopsis] Gateway-adapted 35S vectors for transient expression
(by James.Stark from umb.edu)
Tue Feb 17 01:08:31 EST 2009
I am hoping to find one or more Gateway-adapted 35S vectors to use as effector plasmids in protoplast transient expression assays (PEG transfection). There are many vectors available for Agrobacterium-mediated transformation, but since most are fairly large, smaller plasmids lacking plant selection markers and T-DNA left and right borders would be preferable. Does anyone know of a such a plain 35S-Gateway cassette vector, or perhaps even versions that add epitope tags to the genes inserted in the Gateway sites? Any information or leads would be very much appreciated.
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