[Arabidopsis] RE: Gateway-adapted 35S vectors for transient expression

Xu, Xianfeng via arab-gen%40net.bio.net (by xux from cshl.edu)
Wed Feb 18 14:14:44 EST 2009


Please check out  
pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants
Journal	Plant Molecular Biology
Publisher	Springer Netherlands
ISSN	0167-4412 (Print) 1573-5028 (Online)
Issue	Volume 57, Number 4 / March, 2005

Or there is a pAM PAT GW(35s) vector floating around our lab. It doesn't have any epitope tag. And I've never used it. It's about 7.5kb including the Gateway cassette. Let me know if you are interested.


Morgan (Xianfeng)

Postdoc Fellow
Cold Spring Harbor Laboratory



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Today's Topics:

   1. Gateway-adapted 35S vectors for transient expression 
      (James Stark)
   2. how to check ROS level in plants? (Y Jiang)
   3. BAR news,	Feb. 2009: new interaction data sets and promoter
      tools (Nicholas Provart)

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Message: 1
Date: Tue, 17 Feb 2009 01:08:31 -0500
From: "James Stark" <James.Stark from umb.edu>
Subject: [Arabidopsis] Gateway-adapted 35S vectors for transient
	expression 
To: <arab-gen from magpie.bio.indiana.edu>
Message-ID: <5ACB5AED488C2F44A5B4B8FEA0B0F12203A4EB40 from ebe3.umassb.net>
Content-Type: text/plain;	charset="iso-8859-1"

I am hoping to find one or more Gateway-adapted 35S vectors to use as effector plasmids in protoplast transient expression assays (PEG transfection).  There are many vectors available for Agrobacterium-mediated transformation, but since most are fairly large, smaller plasmids lacking plant selection markers and T-DNA left and right borders would be preferable.  Does anyone know of a such a plain 35S-Gateway cassette vector, or perhaps even versions that add epitope tags to the genes inserted in the Gateway sites?  Any information or leads would be very much appreciated.

Thanks,
Jim Stark
UMass Boston
USA


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