[Arabidopsis] Is overexpression driven by the Gateway vectors weaker?

JH Ahn via arab-gen%40net.bio.net (by fttfl1 from gmail.com)
Thu Jun 11 21:02:40 EST 2009


Dear Arabidopsis netters,

Several experiences in my group suggested that overexpression driven
by the Gateway vectors did not lead to strong effects, comparing to
ones by other vectors, including pCHF3 and pSKI015 (activation tagging
vector). Transcript levels were not greatly increased in transgenic
plants generated by using the Gateway system and in turn only a  small
portion among transgenic plants showed the strong phenotype, seen in
pCHF3-driven or pSKI015-driven plants. Does anyone have similar
experience? Any comments would be much appreciated.

Best regards,

Ji Hoon Ahn
Professor
National Creative Initiative (Lab of Floral Biology)
School of Life Sciences and Biotechnology, Korea University
Anam dong 5 ga, Seongbuk-Gu,
Seoul 136-701, Korea
Phone: 82-2-3290-3451; Fax: 82-2-927-9028
Homepage : http://biotech.korea.ac.kr/lab/PDMG



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