[Arabidopsis] Arabidopsis floral dip

Ramu gowda via arab-gen%40net.bio.net (by ramu.tiger123 from gmail.com)
Fri Mar 20 22:23:16 EST 2009


Dear Rafael Catala
good to know you are working on arabidopsis. i can suggest you to try with
winnans AB medium protocol transformation, other than that you can also try
different agro strains EHA 105, or LBA 4404, we follow majorly these two
strains in inplanta transformation methods. i hope this could be helpfull.
Ramu

On Fri, Mar 20, 2009 at 10:33 PM, <arab-gen-request from oat.bio.indiana.edu>wrote:

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>   1. Transform with JAtY (Hernandez Verdeja, Tamara)
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> Message: 1
> Date: Fri, 20 Mar 2009 17:02:18 +0100
> From: "Hernandez Verdeja, Tamara" <verdeja from inia.es>
> Subject: [Arabidopsis] Transform with JAtY
> To: <Arab-gen from magpie.bio.indiana.edu>
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> Hi everybody,
>
>
>
> I am trying to transform Arabidopsis with a JAtY clone, from the John Innes
> Center Genome Lab and I am not getting any transformant. I am using the
> standard floral-dip protocol with 5% sucrose and the Agrobacterium strain
> C58C1. Could anyone give me some advice? Some specific transformation media,
> Agro strain...
>
>
>
> Thank you very much in advance,
>
>
>
> Rafael Catala
>
>
>
>
>
> Rafael Catala
>
> catala from cib.csic.es
>
> Centro de Investigaciones Biológicas (CIB-CSIC)
> Centre for Biological Research---Spanish Research Council
> Ramiro de Maeztu, 9
> Campus Universidad Complutense
> 28040 Madrid
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> End of Arab-gen Digest, Vol 47, Issue 14
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-- 
Ramu.S.V
Senior research fellow
dept of crop physiology,
Molecular plant physiology lab,
UAS, GKVK, Bangalore


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