[Arabidopsis] Library Amplification Protocol

[Holt, Ben F. III]

Hi all,

My group has several libraries that we would like to amplify in the least biased manner possible. To do this, we would like to use the semi-solid agar method, but we have had some trouble finding a suitable protocol. If anyone has written up a nice lab protocol that you know works well, we would greatly appreciate receiving a copy - any advice you would like to pass on to us is also appreciated.

Thanks in advance!

Ben

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Ben Holt
Assistant Professor
University of Oklahoma
Department of Botany and Microbiology
GLCH Rm 219
770 Van Vleet Oval
Norman, OK  73019
Phone (405)325-9018
FAX   (405)325-7619
http://www.ou.edu/cas/botany-micro/faculty/holt.html