From pwang3 from ualberta.ca Sun Nov 1 20:07:56 2009 From: pwang3 from ualberta.ca (Peng Wang) Date: Sun Nov 1 22:21:07 2009 Subject: [Arabidopsis] The timespan between Agrobacterium infection and active exgenous gene expression Message-ID: <96deb2f40911011707q2ecaa9a6y398726a06e53739e@mail.gmail.com> I am wondering, after infected by Agrobacterium tumefaciens, how soon will the gene in the T-DNA fragment will start to be actively expressed in Arabidopsis. Are there any studies on this topic? Thanks. Ben From shahzadbio from yahoo.com Tue Nov 3 11:31:52 2009 From: shahzadbio from yahoo.com (shahzad anjam) Date: Tue Nov 3 14:40:27 2009 Subject: [Arabidopsis] Regarding your question on Agrobacterium transformation Message-ID: <150839.60449.qm@web36306.mail.mud.yahoo.com> Hi Ben, I am writing regarding your question about Agrobacterium mediated transformation. As I have experienced after 48 hours of co-cultivation, the gene expression starts. As soon as the gene enters in the nucleus its transcription starts. Moreover its further expression depends upon the integration position of gene in the chromosome. I have observed that in gene gun transformation, next day expression was observed. This is my understanding on the topic. Regards Muhammad Shahzad Anjam Lecturer Biotechnology, Institute of Biotechonology , Bahauddin Zakariya University , Multan (www.bzu.edu.pk) Pakistan . Contact Details: Office (Phone) +92(0)61 9210071-4, Ext: 1924, 3900 Cell Phone: +92-333-8174001 E-mail: shahzadanjam@bzu.edu.pk E-mail: shahzadbio@yahoo.com Get your preferred Email name! Now you can @ymail.com and @rocketmail.com. http://mail.promotions.yahoo.com/newdomains/aa/ From staceyg from missouri.edu Tue Nov 3 19:09:14 2009 From: staceyg from missouri.edu (Stacey, Gary) Date: Wed Nov 4 00:45:24 2009 Subject: [Arabidopsis] Assistant Professor Position Available; University of Missouri Message-ID: <964681C84DC6E34F96D993D3717487A707E1229478@UM-EMAIL05.um.umsystem.edu> UNIVERSITY of MISSOURI Position Description: The Division of Plant Sciences, University of Missouri, invites applications for a tenure-track position in plant cell wall biology. The 12-month, assistant professor position is 60% research and 40% teaching. Responsibilities: The successful candidate will be expected to establish an innovative, extramurally funded research program on the biology, development, and degradation of plant cell walls and associated ligno-cellulosic structures. Research should focus on utilization of cellulosic compounds by the emerging biofuels industries and by economically important food-animal (livestock) species. The University of Missouri has a long history of collaboration within the Division of Plant Sciences and with other teams of scientists across campus. The candidate will be expected to explore collaborative opportunities with the Interdisciplinary Plant Group, the University of Missouri Center for Sustainable Energy, and teams of scientists working on thermo-chemical conversion of biomass, pasture-based dairies, and the forage-based beef industry. Accessible to the candidate are excellent laboratory and field research facilities (both on and off-campus research centers) within the Missouri Agricultural Experiment station network. The successful candidate is expected to participate in the undergraduate and graduate teaching and service missions of the Division of Plant Science. Teaching responsibilities include an undergraduate forage crops class, as well as a new course in bio-energy crops. Future teaching opportunities could include self or team-taught graduate courses in the candidate's area of expertise. Qualifications: The applicant must have a Ph.D. degree in Crop Science, Agronomy, Plant Sciences or related field. Effective oral and written communications skills are required. Training in the development, degradation, biochemistry, or physiology of cell walls as it relates to the biofuels and/or food-animal industries is desirable. Post-doctoral experience is also desirable. Applications: Applicants should electronically submit to PlantSci@missouri.edu the following items in MSWord or PDF format: a letter of application, a curriculum vitae, transcripts, a list of four references and their contact information, and reprints of major publications. Hard copies of required materials can be sent to Christa Smith, ESA Division of Plant Sciences 1-41 Agriculture Bldg. University of Missouri Columbia, MO 65211. Questions regarding this position should be directed to Dr. Rob Kallenbach, Chair, Search Committee, at kallenbachr@missouri.edu or 573-884-2213. Job Description #: 1506453; OARS file # 090218 Salary and Benefits: Benefits available include health, dental, life and disability insurance and a retirement plan. Salary will be commensurate with qualifications and experience. Application Deadline: Review of applications will begin February 1, 2010 and will continue until a qualified candidate is identified. The University of Missouri-Columbia is an Equal Opportunity/Affirmative Action employer. To request ADA accommodations, please contact our ADA coordinator. (V/TTY). Visit the University of Missouri-Columbia's web site a Gary Stacey, Ph.D. Director, Center for Sustainable Energy Assoc. Director, National Center for Soybean Biotechnology Divisions of Plant Sciences and Biochemistry Department of Molecular Microbiology and Immunology 271E Christopher S. Bond Life Sciences Center University of Missouri Columbia, MO 65211 Office phone: 573-884-4752 Lab Phone: 573-884-4799 FAX: 573-884-9676 email: staceyg@missouri.edu NEW LAB WEBSITE: http://www.staceylab.missouri.edu/ MU CSE site: http://www.energy.missouri.edu/ NCSB site: http://www.soybiotechcenter.org NSF Project site: http://www.soybeangenome.org From Jean-Luc.Gallois from avignon.inra.fr Wed Nov 4 02:14:38 2009 From: Jean-Luc.Gallois from avignon.inra.fr (Gallois) Date: Wed Nov 4 12:34:07 2009 Subject: [Arabidopsis] looking for pBI221 Message-ID: <4AF129DE.4000101@avignon.inra.fr> Dear colleagues, I am looking for an old plasmid, pBI221 (if I recall well, a pUC-based plasmid with a 35Spromoter-GUS-Nosterm construct in it). It used to be sold by Clontech, in the old days.If anyone has some left and could spare me an aliquot, I would be grateful. Regards Jean-luc -- Jean-Luc GALLOIS INRA-UR 1052 G?n?tique et Am?lioration des Fruits et L?gumes Domaine St Maurice, BP94 84143 Montfavet cedex, France tel : +00 33 (0)4 32 72 27 96 e-mail : jlgallois@avignon.inra.fr From eb50 from nyu.edu Thu Nov 5 10:09:55 2009 From: eb50 from nyu.edu (Eric D Brenner) Date: Thu Nov 5 16:04:02 2009 Subject: [Arabidopsis] Arabidopsis in teaching intro. biology Message-ID: <5f00e3b437e5de.4af2a473@mail.nyu.edu> Dear Colleagues, I would like to know if any of you use Arabidopsis in introductory biology teaching courses? If so, would you be so kind as to share with me the experiment that you perform, the strains/mutants used, and a general idea in how long the experiment takes, and the source and cost of materials? Thank you ever so kindly, Eric D. Brenner, PhD. Clinical Assistant Professor Department of Biology New York University 609 Silver Building 100 Washington Square East New York, NY 10003 wk: 212-992-8693 fx: 212-995-4204 From h.poorter from gmail.com Fri Nov 6 12:29:55 2009 From: h.poorter from gmail.com (Hendrik Poorter) Date: Sun Nov 8 23:19:43 2009 Subject: [Arabidopsis] Re: Arab-gen Digest, Vol 55, Issue 4 In-Reply-To: <200911061703.nA6H3jM06372@net.bio.net> References: <200911061703.nA6H3jM06372@net.bio.net> Message-ID: In reply to the question of Eric Brenner about teaching and Arabidopsis in intro biology: At Utrecht University we have a one week course (divided over 2 weeks) on the experimental cycle. Students have to follow this cycle almost full circle. We pregrow a number of Arabidopsis mutants (sex-1 of course, always a favourite, pgm, but also a few hormone mutants) and their WT for them in the growth chamber, in pots, at 2 light intensities. The measurements they have to do are necessarily fixed already beforehand, but they have to find information about their mutant on the web and in the literature, and then they have to formulate the exact question and the hypothesis they have, both about the effect of light and of the mutation. Then they measure traits at 3 levels: gene expression of Rubisco and a CAB gene by qPCR, Rubisco and chlorophyll content, and specific leaf area, along with dry weight:fresh weight ratio's, leaf size and shape, leaf angle and what they like to measure more. They have to calculate a number of variables and enter them all on papers with predesigned tables with the relevant traits. In this way we can see whether relative differences in gene expression translate into a differences in the Chlorophyll:Rubisco ratio, and how these changes relate to differences in SLA, or concentration per unit fresh or dry weight. They have to draw their conclusions, make a poster and present these for other students of their year. Great fun! Success, Hendrik Poorter On Fri, Nov 6, 2009 at 6:03 PM, wrote: > Send Arab-gen mailing list submissions to > arab-gen@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/arab-gen > or, via email, send a message with subject or body 'help' to > arab-gen-request@net.bio.net > > You can reach the person managing the list at > arab-gen-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Arab-gen digest..." > > > Today's Topics: > > 1. Arabidopsis in teaching intro. biology (Eric D Brenner) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 05 Nov 2009 10:09:55 -0500 > From: Eric D Brenner > Subject: [Arabidopsis] Arabidopsis in teaching intro. biology > To: arab-gen@magpie.bio.indiana.edu > Message-ID: <5f00e3b437e5de.4af2a473@mail.nyu.edu> > Content-Type: text/plain; charset=us-ascii > > Dear Colleagues, > > I would like to know if any of you use Arabidopsis in introductory biology > teaching courses? If so, would you be so kind as to share with me the > experiment that you perform, the strains/mutants used, and a general idea in > how long the experiment takes, and the source and cost of materials? > > Thank you ever so kindly, > > Eric D. Brenner, PhD. > Clinical Assistant Professor > Department of Biology > New York University > 609 Silver Building > 100 Washington Square East > New York, NY 10003 > wk: 212-992-8693 > fx: 212-995-4204 > > > > > ------------------------------ > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > End of Arab-gen Digest, Vol 55, Issue 4 > *************************************** > -- Dr. Hendrik Poorter ICG-3; Research Institute J?lich D-52428 J?lich; Germany tel: +49-(0)2461-61-6833 www.science.poorter.eu From neil from arabidopsis.info Fri Nov 6 04:05:29 2009 From: neil from arabidopsis.info (Neil Graham) Date: Sun Nov 8 23:20:08 2009 Subject: [Arabidopsis] Affymetrix tobacco array Message-ID: <83E0831E-3CEB-4499-B87D-E7FE423CBAC4@arabidopsis.info> Dear All The NASC Affymetrix service is now able to offer the tobacco GeneChip. This has been developed by Adavnced Technologies Cambridge (ATC) and contains probe-sets for > 43,000 tobacco sequences (http://www.bat-science.com/groupms/sites/BAT_7AWFH3.nsf/vwPagesWebLive/DO7LTLWC?opendocument&SKN=2 ). The array is being offered as part of our normal full Affymetrix service, which includes RNA QC, labeling, hybridisation and data delivery. Assistance can also be provided with experimental design and data analysis. All the data generated from this array will be made publicly available through the NASCArrays database. The cost is currently ?450 (+VAT) per array. The array could also be used with our Xspecies technique (http://affymetrix.arabidopsis.info/xspecies/ ) to study related species. For more information on the NASCArrays service please see: http://affymetrix.arabidopsis.info/ or email affy@arabidopsis.info Best Wishes Neil Graham -------------------------------------------------- NASC Plant and Crop Sciences Division University of Nottingham Sutton Bonington Campus Loughrough LE12 5RD www.arabidopsis.info ------------------------------------------------- This message has been checked for viruses but the contents of an attachment may still contain software viruses, which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. From ramu.tiger123 from gmail.com Fri Nov 6 09:40:11 2009 From: ramu.tiger123 from gmail.com (Ramu gowda) Date: Sun Nov 8 23:20:34 2009 Subject: [Arabidopsis] Re: Arab-gen Digest, Vol 55, Issue 3 In-Reply-To: <200911051704.nA5H4KM06647@net.bio.net> References: <200911051704.nA5H4KM06647@net.bio.net> Message-ID: Dear Jean-Luc GALLOIS are you asking for pBI121. if so yes we have that but how do i send to you. let me know the details ramu On Thu, Nov 5, 2009 at 10:34 PM, wrote: > Send Arab-gen mailing list submissions to > arab-gen@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/arab-gen > or, via email, send a message with subject or body 'help' to > arab-gen-request@net.bio.net > > You can reach the person managing the list at > arab-gen-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Arab-gen digest..." > > > Today's Topics: > > 1. looking for pBI221 (Gallois) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 04 Nov 2009 08:14:38 +0100 > From: Gallois > Subject: [Arabidopsis] looking for pBI221 > To: arab-gen@magpie.bio.indiana.edu > Message-ID: <4AF129DE.4000101@avignon.inra.fr> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Dear colleagues, > I am looking for an old plasmid, pBI221 (if I recall well, a pUC-based > plasmid with a 35Spromoter-GUS-Nosterm construct in it). It used to be > sold by Clontech, in the old days.If anyone has some left and could > spare me an aliquot, I would be grateful. > Regards > Jean-luc > > -- > Jean-Luc GALLOIS > INRA-UR 1052 > G?n?tique et Am?lioration des Fruits et L?gumes > Domaine St Maurice, BP94 > 84143 Montfavet cedex, France > tel : +00 33 (0)4 32 72 27 96 > e-mail : jlgallois@avignon.inra.fr > > > > > ------------------------------ > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > End of Arab-gen Digest, Vol 55, Issue 3 > *************************************** > -- Ramu.S.V Research Associate COE, DBT Molecular plant physiology lab, dept of crop physiology, UAS, GKVK, Bangalore From dee.rawsthorne from bbsrc.ac.uk Mon Nov 9 06:23:22 2009 From: dee.rawsthorne from bbsrc.ac.uk (dee rawsthorne (TOC)) Date: Mon Nov 9 13:27:26 2009 Subject: [Arabidopsis] Five posts at UEA and a Project Leader position at John Innes Centre Message-ID: <92C274E2DE278B4EBAAF9DA3CB0DE2900490CDFD21@nbiexch1.nbi.bbsrc.ac.uk> Five new academic staff appointments at UEA and a Project Leader at the John Innes Centre have been created as a result of substantial new investments in the Earth and Life Systems Alliance (ELSA) and the Tyndall Centre for Climate Change Research. The posts offer excellent opportunities for continuing, or developing, internationally outstanding research careers. The Tyndall Centre for Climatic Change Research Three Lecturer Posts Ref: ATR842 (?37,651 to ?43,622 per annum) UEA is the Headquarters of the Tyndall Centre (www.tyndall.ac.uk), which includes the universities of Manchester, Southampton, Oxford, Newcastle, Sussex and Cambridge. At UEA, the Tyndall Centre's interdisciplinary activities span the Faculty of Science and the Faculty of Social Sciences. These new positions will be based alongside existing Tyndall colleagues within the School of Environmental Sciences. You must have a PhD (or equivalent) in an appropriate discipline in physical, natural, or social science, ideally with research interests in one or more of: land use and climate change; food security and climate change; mitigation, adaptation and behavioural change; energy and climate security; climate change and prosperity, and you must also be able to satisfy all other essential elements of the person specification. Applications from candidates with research interests in other climate-related areas will also be considered. The Earth and Life Systems Alliance (ELSA) Two UEA Lecturer or Reader posts Ref: ATR841 (?37,651 to ?43,622 per annum for lecturer, ?44,930 to ?52,086 for reader) John Innes Centre Project Leader (Tenure-track) Ref: 1001841 (?33,780 - ?37,534. A higher pay band (?42,769 to ?47,521) may be offered to an exceptional candidate). ELSA (www.elsa.ac.uk) is a major strategic collaboration between UEA and the John Innes Centre (JIC). It brings together UEA's expertise in environmental science and climate change, with JIC's expertise in plant and microbial biology to understand, predict and mitigate the impact of environmental change on biodiversity, biogeochemical cycles and agricultural systems. We seek applicants with outstanding research and publication records in this area. The successful candidates will either have or be expected to establish an internationally high-profile independent research programme that includes substantial collaboration between JIC and UEA. For all ELSA posts, you must have a PhD (or equivalent); a research background in ecology, molecular or evolutionary biology, biogeochemistry, microbiology, plant biology, social science or environmental modelling and be able to satisfy all other elements of the person specification. Successful candidates will have a developing international research reputation, which will already be established for appointment to the higher level. Closing date: 12 noon on 30 November 2009. http://www.uea.ac.uk/hr/jobs/acad/atr841.htm Professor Alastair Grant Deputy Head of School School of Environmental Sciences University of East Anglia, Norwich, NR4 7TJ, UK Phone +44 1603 592537 From kristina.rizzardi from ebc.uu.se Tue Nov 10 12:20:52 2009 From: kristina.rizzardi from ebc.uu.se (Kristina Rizzardi) Date: Tue Nov 10 13:19:47 2009 Subject: [Arabidopsis] PhD position availible in plant physiology Message-ID: <20091110182052.ugb55jqm7k8ck8cs@webmail6.uu.se> Uppsala University hereby declares the following position to be open for application: PhD student position in Physiological Botany at the Evolutionary Biology Centre, the Department of Physiological Botany (http://www.ebc.uu.se) announces a PhD student position in the project ?The role of the Arabidopsis HETEROCHROMATIN PROTEIN1 in plant development?. In plants as well as in animals the control of chromatin structure in response to endogenous and exogenous cues determines gene expression patterns that regulate cell differentiation and development. The available project focuses on an Arabidopsis mutant, terminal flower2 (tfl2). The gene encodes the plant HETEROCHROMATIN PROTEIN1 (HP1), a homologue to the Drosophila HP1 genes that are involved in basic processes as DNA replication, recombination and repair but also in epigenetics and gene activation/repression during differentiation. The single copy gene in Arabidopsis encodes a protein most similar to HP1 proteins that regulate gene activation/ repression in other organisms and it has been shown to localize to euchromatic regions of the chromosomes. Mutations in tfl2 have a pleiotropic effect indicating a global role, possibly regulating different developmental processes in response to light. Our data points to the importance of TFL2 in two phase-transitions during the plant life cycle: embryo ? postembryonic development as well as vegetative ? reproductive development. Recent results show that TFL2 physically interact with proteins in hormone signal transduction pathways as well as chromatin of both hormone biosynthesis and response genes. The successful candidate will collaborate with another PhD student in this area of hormone regulation of plant development. The successful candidate should have a university degree in biology, favourably with the combination of plant biology and molecular biology. The application should include personal background including a description of undergraduate training, a complete CV, copies of university degree and degree thesis as well as the names and e-mail addresses to two referees. The PhD position is full time employment position for a tenure of four years. However, the possibility for the tenure to be extendable to a maximum of five years exists to allow the inclusion of university level biology teaching and/or laboratory supervision into the PhD education. For more information please contact Dr Annika Sund?s-Larsson, that will function as the supervisor, by e-mail annika.sundas-larsson@ebc.uu.se or by phone +46-18-4712819. Union representatives are Anders Grundstr?m, SACO-r?det, tel: +46 18-471 5380, Carin S?derh?ll, TCO/ST, tel: +46 18-471 1996 and Stefan Djurstr?m, SEKO, tel: +46 18-471 3315. The application should be sent , preferrably by e-mail to: registrator@uu.se, or by fax + 46 18 471 2000, or by mail to: Registrar?s Office, Uppsala University, Box 256, SE-751 05 UPPSALA, Sweden. In any correspondence, please use the reference number UFV-PA 2009/2423. Application no later than 16 November, 2009 From ecolik12 from gmail.com Fri Nov 13 18:03:07 2009 From: ecolik12 from gmail.com (jay shah) Date: Sat Nov 14 16:12:53 2009 Subject: [Arabidopsis] Growing Arabidopsis in vermiculite Message-ID: <11acc0af0911131503s39c1730dqa8d12494aaed8abd@mail.gmail.com> Hello all, I would like to grow Arabidopsis in a way that i should not have any micro organisms in the substrate in which i am growing them. Can i use vermiculite instead of soil? is there any other means in which i can grow the plants so that i have no microbial contamination in the growing environment, specially roots? I would like to measure certain compounds, which in presence or micro organisms can create an error in the analysis. Regards, -- Jay Shah. TOUGH TIMES NEVER LAST, TOUGH PEOPLE DO From ramu.tiger123 from gmail.com Mon Nov 16 04:07:49 2009 From: ramu.tiger123 from gmail.com (Ramu gowda) Date: Mon Nov 16 13:34:35 2009 Subject: [Arabidopsis] Re: Arab-gen Digest, Vol 55, Issue 8 In-Reply-To: <200911151704.nAFH4FM27905@net.bio.net> References: <200911151704.nAFH4FM27905@net.bio.net> Message-ID: Dear Jay Shah you can grow the arabidopsis in an quartz sand if you are perticularly interested to study the roots. while using quartzsand you autoclave and use and every two or 3 days once you can add half MS or Hogland solution. the quartz sand allow roots to grow with out any difficulty . hope you may find it easy. wish success. On Sun, Nov 15, 2009 at 10:34 PM, wrote: > Send Arab-gen mailing list submissions to > arab-gen@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/arab-gen > or, via email, send a message with subject or body 'help' to > arab-gen-request@net.bio.net > > You can reach the person managing the list at > arab-gen-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Arab-gen digest..." > > > Today's Topics: > > 1. Growing Arabidopsis in vermiculite (jay shah) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 13 Nov 2009 19:33:07 -0330 > From: jay shah > Subject: [Arabidopsis] Growing Arabidopsis in vermiculite > To: arab-gen@magpie.bio.indiana.edu > Message-ID: > <11acc0af0911131503s39c1730dqa8d12494aaed8abd@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hello all, > > I would like to grow Arabidopsis in a way that i should not have any micro > organisms in the substrate in which i am growing them. > > Can i use vermiculite instead of soil? > > is there any other means in which i can grow the plants so that i have no > microbial contamination in the growing environment, specially roots? I > would > like to measure certain compounds, which in presence or micro organisms can > create an error in the analysis. > > Regards, > > -- > Jay Shah. > > TOUGH TIMES NEVER LAST, TOUGH PEOPLE DO > > > ------------------------------ > > _______________________________________________ > Arab-gen mailing list > Arab-gen@net.bio.net > http://www.bio.net/biomail/listinfo/arab-gen > > End of Arab-gen Digest, Vol 55, Issue 8 > *************************************** > -- Ramu.S.V Research Associate COE, DBT Molecular plant physiology lab, dept of crop physiology, UAS, GKVK, Bangalore From loudet from versailles.inra.fr Tue Nov 17 20:39:40 2009 From: loudet from versailles.inra.fr (Olivier Loudet) Date: Tue Nov 17 22:59:35 2009 Subject: [Arabidopsis] 4 Post-Doc + 3 Lab. Asst positions at INRA Versailles, France / Loudet' lab Message-ID: <6.2.1.2.2.20091118023857.028dcc40@versailles.inra.fr> We are looking for 4 highly motivated post-doc candidates to work on Arabidopsis natural variation for growth and the response to the abiotic environment, as well as cis-acting regulation of gene expression. Also, 3 lab. assistant positions available. More information and contacts available by following this link (pdf): http://www.inra.fr/vast/Positions_2010_Loudet_INRAVersailles.pdf These 2-3 years fellowships are available at INRA, Versailles (France), in the VAST lab. Please forward this offer to your network and students. O. ________________________________________________________ Olivier Loudet INRA, National Institute for Agricultural Research Versailles, France V.A.S.T group -- Variation and Abiotic Stress Tolerance Mail: loudet@versailles.inra.fr Science: www.inra.fr/vast MyWeb: olivier.loudet.net MyOwnGenes: teva.loudet.net & laena.loudet.net " Catch your Fire in your Turn and Let it Burn " ________________________________________________________ From anilmalik58 from gmail.com Wed Nov 18 08:04:09 2009 From: anilmalik58 from gmail.com (anilmalik58@gmail.com) Date: Wed Nov 18 15:06:37 2009 Subject: [Arabidopsis] Arabidopsis Message-ID: <1c95f62f-8411-4209-9825-753d41078cd9@m33g2000pri.googlegroups.com> dear sir i want to infect Arabidopsis thaliana using nematode (M.incognita) .can please guide to know that which mixture(soiil,sand vermiculite and cocopete) is best for plant growth as well as for nematode holding capacity. i am hopinbg your reply. From beth.rueschhoff from gmail.com Wed Nov 18 10:31:28 2009 From: beth.rueschhoff from gmail.com (Beth Rueschhoff) Date: Wed Nov 18 15:09:10 2009 Subject: [Arabidopsis] Looking for a good GR-inducible vector with a GFP Tag Message-ID: <84c4c8a50911180731h5b7d83aj6cd6e6915937d8f7@mail.gmail.com> Greetings all! I am looking for a GR inducible vector that also has a tag. Preferably, the tag would be GFP, but I could use something else if need be. Does anyone have experience with a good vector? Thanks in advance for suggestions! Beth Rueschhoff, Ph.D. Department of Genetics NC State University From nicholas.provart from utoronto.ca Thu Nov 19 18:09:59 2009 From: nicholas.provart from utoronto.ca (Nicholas Provart) Date: Thu Nov 19 19:31:32 2009 Subject: [Arabidopsis] BAR News: new eFP Browser cell-type-specific data sets and views for other species, and protein structures in ePlant Message-ID: <200911192310.nAJNASM14360@net.bio.net> Hello Arabidopsis and other Plant Researchers, The Bio-Array Resource is pleased to announce the inclusion of several new cell-type-specific expression data sets in our Arabidopsis eFP Browser at http://bar.utoronto.ca/efp. These cell-type-specific data sets in the Tissue Specific view include: -a floral meristem set from Venu Reddy's lab at UCR; -pollen germination data from Mark Johnson (Brown) and Ravishankar Palanivelu (Arizona); and in a new Root view: -salt and iron-deficiency root cell-type-response data from the Benfey Lab (Duke); -high-resolution root spatiotemporal expression data, again from the Benfey Lab; -nitrogen root cell-type-responses from the Birnbaum Lab (NYU); Many thanks to Siobhan Brady, Benfey lab members, Venu Reddy, Mark Johnson and others for input in the creation of these eFP views. The Root view alone provides a snapshot of 196 different combinations of cell-types versus environmental responses or developmental stages (xylem and phloem pole pericycle, procambium, xylem, phloem, phloem companion cells and other cell types in 13 different sections generated by Dustin Cartwright and Hardeep Nahal using an EM algorithm from Cartwright et al. 2009: try it out with PIN1, At1g73590, as a nice example!), from 268 data sets across 6.1 million data points. We're also premiering eFP Browsers for barley and rice in beta - find the links to these on the BAR homepage at http://bar.utoronto.ca. In addition, it is now possible to explore Poplar data from the Campbell Lab's PopGenExpress data sets using our Expression Angler and Expression Browser tools. The links are also on the BAR homepage. Finally, our ePlant now contains predicted protein structures for approximately 72% of the Arabidopsis proteome, based on work carried out in collaboration with Lawrence Kelley at Imperial College London using his Phyre algorithm. Check it out at http://bar.utoronto.ca/eplant. Feedback as always is welcome! Best regards, Nick ......................................... Nicholas Provart, PhD Assistant Professor, Plant Cyberinfrastructure & Systems Biology Director, Graduate Program in Genome Biology and Bioinformatics Member, Centre for the Analysis of Genome Evolution and Function Rm 3051, Dept. of Cell and Systems Biology, Uni. Toronto 25 Willcocks St., Toronto, ON. M5S 3B2. CANADA Tel. (+1) (416) 978-7141, Fax. (+1) (416) 978-5878 URL. http://www.csb.utoronto.ca/faculty/provart-nicholas The Bio-Array Resource. http://www.BAR.utoronto.ca email. nicholas.provart@utoronto.ca TTC. Spadina LRT, Willcocks St. From heisler from embl.de Mon Nov 23 03:56:56 2009 From: heisler from embl.de (Marcus Heisler) Date: Mon Nov 23 16:11:29 2009 Subject: [Arabidopsis] TurboYFP in plants/Arabidopsis? Message-ID: Hi. I am wondering whether anyone has tried TurboYFP from Evrogen in plants yet. We just tried the related PhiYFP and could not detect any expression in Tobacco transients. Best wishes, Marcus. Dr Marcus Heisler Developmental Biology Unit EMBL Heidelberg Meyerhofstrasse 1 69117 Heidelberg Germany email: heisler@embl.de phone: +49 6221 3878603 From huala from acoma.stanford.edu Mon Nov 23 14:45:03 2009 From: huala from acoma.stanford.edu (Eva Huala) Date: Mon Nov 23 16:12:12 2009 Subject: [Arabidopsis] Nature News article on TAIR funding cuts Message-ID: <4B0AE63F.6080105@acoma.stanford.edu> Dear colleagues, I'd like to draw your attention to the Nature News article on TAIR funding cuts that appeared in last Thursday's issue. The news article includes a comment box at the bottom where you can voice your opinion on the cuts or add suggestions on what could be done to solve this problem: http://www.nature.com/news/2009/091118/full/462258b.html A substantial number of comments on this piece will help make the case that the community cares about this issue. Best, Eva Huala TAIR Director From schumake from Ag.arizona.edu Sun Nov 22 13:38:10 2009 From: schumake from Ag.arizona.edu (Karen Schumaker) Date: Mon Nov 23 16:12:47 2009 Subject: [Arabidopsis] Postdoctoral position available Message-ID: <005201ca6ba2$f14586e0$d3d094a0$@arizona.edu> I am writing to ask that the advertisement for a postdoctoral position in my laboratory included below be posted on your site. I would be happy to answer any questions. Thank you in advance for your help. Karen S. Schumaker Departments of Plant Sciences and Molecular and Cellular Biology and The BIO5 Institute University of Arizona Forbes 303 Tucson, AZ 85721 (520) 621-9635 http://www.ag.arizona.edu/research/schumaker/ __ A postdoctoral associate position is available at the University of Arizona as part of a Department of Energy-funded project to study endogenous programs that control plant growth and development and how those programs are altered to enable the plant to grow in non-optimal conditions (http://www.ag.arizona.edu/research/schumaker/). A major focus of our work is to understand the roles of the Calcineurin B-Like calcium binding proteins in the establishment of developmental specificity during calcium-mediated signaling in Arabidopsis. We are looking for a highly motivated individual with a Ph.D. degree and a demonstrated ability to carry out outstanding research in the fields of plant development, genetics or related disciplines. Proven experience with molecular biology techniques and demonstrated written and oral communication skills are essential qualifications. Ability to interact and work collaboratively with others is also critical. The position is available February 1, 2010. To apply, send a curriculum vitae and the names and contact information (address, phone, fax and e-mail) for three references to Karen Schumaker, Ph.D. (schumake@ag.arizona.edu), School of Plant Sciences, University of Arizona, Tucson, Arizona 85721-0036, United States. FAX 520-621-7186. The University of Arizona is an EEO/AA Employer-M/W/D.V. From benholt from ou.edu Tue Nov 24 12:19:30 2009 From: benholt from ou.edu (Holt, Ben F. III) Date: Tue Nov 24 15:09:55 2009 Subject: [Arabidopsis] Vector NTI Replacement Message-ID: Hi All, I am curious to hear what others are using for managing and manipulating their DNA sequences on the computer. We have used VectorNTI for years, but now that they have switched to the annual fee, we would like to consider alternatives. Thoughts? Ben ======== Ben Holt Assistant Professor University of Oklahoma Department of Botany and Microbiology GLCH Rm 219 770 Van Vleet Oval Norman, OK 73019 Phone (405)325-9018 FAX (405)325-7619 http://www.ou.edu/cas/botany-micro/faculty/holt.html