[Arabidopsis] Regarding your question on Agrobacterium transformation

[shahzad anjam]

Hi Ben,

I am writing regarding your question about Agrobacterium mediated transformation. As I have experienced after 48 hours of co-cultivation, the gene expression starts. As soon as the gene enters in the nucleus its transcription starts. Moreover its further expression depends upon the integration position of gene in the chromosome. I have observed that in gene gun transformation, next day expression was observed.
This is my understanding on the topic.
 
 Regards
 
Muhammad Shahzad Anjam 
Lecturer Biotechnology, Institute of Biotechonology , 
Bahauddin Zakariya University , Multan (www.bzu.edu.pk)
Pakistan . 


Contact Details: 
Office (Phone) +92(0)61 9210071-4, Ext: 1924, 3900
Cell Phone: +92-333-8174001
E-mail: shahzadanjam from bzu.edu.pk 
E-mail: shahzadbio from yahoo.com 



      Get your preferred Email name!
Now you can @ymail.com and @rocketmail.com. 
http://mail.promotions.yahoo.com/newdomains/aa/