[Arabidopsis] Regarding your question on Agrobacterium
(by shahzadbio from yahoo.com)
Tue Nov 3 11:31:52 EST 2009
I am writing regarding your question about Agrobacterium mediated transformation. As I have experienced after 48 hours of co-cultivation, the gene expression starts. As soon as the gene enters in the nucleus its transcription starts. Moreover its further expression depends upon the integration position of gene in the chromosome. I have observed that in gene gun transformation, next day expression was observed.
This is my understanding on the topic.
Muhammad Shahzad Anjam
Lecturer Biotechnology, Institute of Biotechonology ,
Bahauddin Zakariya University , Multan (www.bzu.edu.pk)
Office (Phone) +92(0)61 9210071-4, Ext: 1924, 3900
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E-mail: shahzadbio from yahoo.com
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