[Arabidopsis] endogenous control for ABI Taqman and SYBR green dyes

manoj ruhal via arab-gen%40net.bio.net (by ruhal.manoj from gmail.com)
Wed Sep 2 14:43:11 EST 2009


 Hello everyone,
I am trying to get relative standard curve of plant myosin gene. So in
real-time PCR I am using SYBR green PCR master mix to get fluorescence.I
have ABI Taqman human GAPDH control as an endogenous control in place of
SYBR .For endogenous control, Is it possible to use ABI Taqman human GAPDH
assay mix with SYBR chemistry?-
Kumar, Manoj
Research Assistant
Microscopy Center
Department of Biology
University of Louisiana at Lafayette
Lafayette, LA 70504 USA


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