[Arabidopsis] linkage inheritance

Yin, Ruohe via arab-gen%40net.bio.net (by ruohe.yin from helmholtz-muenchen.de)
Wed Sep 30 03:42:19 EST 2009

Dear all,

I have two T-DAN insertion Arabidopsis lines. The two knock-out loci are about 1Mb in distance on chromosome V.
After genetic cross of the two single mutants, F1 plants were self-pollinated and seeds were harvested.
My question is how many F2 individuals I would need to ensure one expected double mutant.
Thanks a lot for your help.


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