[Arabidopsis] Protein localization

Wolf Frommer via arab-gen%40net.bio.net (by wfrommer from stanford.edu)
Mon Apr 19 14:10:24 EST 2010


We have successfully used rdr6 or sgs3 mutants to reduce effects caused by silencing (cf. Deuschle et al., 2006 Plant Cell).
On Apr 19, 2010, at 11:42 AM, Peng, Jianling wrote:

> 
> Hi everyone,
> 
> I am studying the subcellular localization of my interested protein. I
> made several GFP and mCherry fusion construct and transformed them into
> Arabidopsis. Unfortunately, none of them gave me any flurescence.
> Construct is in frame for sure. Anybody has any experience? Any
> suggesions and comments are appreciated.
> 
> Thanks!
> 
> Jianling
> 
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**************************************
Wolf B. Frommer
Carnegie Institution for Science
Department of Plant Biology
260 Panama St
Stanford
CA 94305
Tel. 650 325 1521 x208
http://carnegiedpb.stanford.edu/
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