[Arabidopsis] Re: Protein localization
(by temnik from rambler.ru)
Tue Apr 20 12:24:10 EST 2010
Have you tried to transiently express your fusion constructs in
N.benthamiana first? Check them after 1, 2, 3 and 4 days post
inoculation to see if you can detect any fluorescence. Also do Western
with the antiGFP antibodies to check if you hybrid protein
accummulates at all.
Peng, Jianling wrote:
> Hi everyone,
> I am studying the subcellular localization of my interested protein. I
> made several GFP and mCherry fusion construct and transformed them into
> Arabidopsis. Unfortunately, none of them gave me any flurescence.
> Construct is in frame for sure. Anybody has any experience? Any
> suggesions and comments are appreciated.
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