[Arabidopsis] Seed protein extraction and IP

[Paula Duque]

Hello,

We are wanting to conduct immunoprecipitation with anti-HA antibodies  
from imbibed Arabidopsis seeds following formaldehyde fixation  
(crosslinking). Does anyone know of adequate protocols or have useful  
tips?

Many thanks in advance.
Cheers,

Paula

Paula Duque
Laboratory of Plant Molecular Biology
Instituto Gulbenkian de Ciência
Rua da Quinta Grande, 6
2780-156 Oeiras
Portugal

Phone: +351 214464630
Fax: +351 214407970
Email: duquep from igc.gulbenkian.pt