[Arabidopsis] Re: Arab-gen Digest, Vol 63, Issue 9

[Ignacio Rubio]

It is perfectly possible if the GFP is under a promoter already. I have done it for several control lines. I hope that helps.
Nacho

On Sun, 25 Jul 2010 12:03:14 -0500 (EST)
 arab-gen-request from oat.bio.indiana.edu wrote:
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>    1. Is it possible to transform empty Gateway vectors	into
>       Arabidopsis? (Guan Yue-Feng)
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> Message: 1
> Date: Sat, 24 Jul 2010 08:14:39 -0500
> From: "Guan Yue-Feng" <guanyf from gmail.com>
> Subject: [Arabidopsis] Is it possible to transform empty Gateway
> 	vectors	into Arabidopsis?
> To: "arab-gen" <arab-gen from magpie.bio.indiana.edu>
> Message-ID: <201007240814371252656 from gmail.com>
> Content-Type: text/plain;	charset="us-ascii"
> 
> Hi All,
> I am doing protein fusion with GFP in Gateway vectors to study the subcellular localization.  For control, can I transform the empty vectors into Arabidopsis without modification?
> If it is not, is there a simple way to remove the ccdB cassette?
> I tried transformation of Agro with the vectors, and appreared to get positive colonies.
> Thanks
>  				
> --------------
> Guan Yue-Feng
> 2010-07-24
> 
> 
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> End of Arab-gen Digest, Vol 63, Issue 9
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------------------------------------------------------------

Ignacio Rubio Somoza PhD

Max Planck Institute for Developmental Biology
Department of Molecular Biology (VI)
Spemannstr. 37-39
D-72076, Tübingen, Germany
phone: +49 (0)7071 601 1405