[Arabidopsis] vertical growth

Brian Cady via arab-gen%40net.bio.net (by briancady101 from yahoo.com)
Thu Nov 18 12:11:04 EST 2010


Here at Adan Colon-Carmona's lab we wrap bundles of 3-6 square plates with a 
rubber band. When banded together, the unit will sit in the incubator with each 
plate vertically aligned.

Brian Cady





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Subject: Arab-gen Digest, Vol 67, Issue 7

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Today's Topics:

   1. Re: Petri Dish Racks (Tobias Baskin)
   2. Re: Petri Dish Racks (Holt, Ben F. III)
   3. Re: Petri Dish Racks (Rosemary White)


----------------------------------------------------------------------

Message: 1
Date: Wed, 17 Nov 2010 08:19:57 -0500
From: Tobias Baskin <baskin from bio.umass.edu>
Subject: Re: [Arabidopsis] Petri Dish Racks
To: SaraN <passiflora42 from yahoo.com>
Cc: arab-gen from magpie.bio.indiana.edu
Message-ID: <p06240504c90985ad185f@[10.0.1.2]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Hi,
    We made our own by hand, lots of little strips of plexi 
glass. Its a pain. Its a bit easier to make them from wood, but still 
tedious. I have since learned that some baking cooling racks have 
spacings that are just right. You will have an amusing trip to the 
local supermarket or home-stuff store with your petri dish to find a 
product with optimal spacing. I have also seen plates placed all 
together in small boxes. This doesn't give space between each plate 
but if the boxes have shallow sides (like pipette tip boxes) it can 
be ok. Hope this helps.
            Tobias Baskin

>Does anyone know of a product that would hold petri dish racks
>vertically?  We are growing Arabadopsis on plates in various solutions
>and need them to be vertical so they grow upright.  Thanks in advance.
>
>_______________________________________________
>Arab-gen mailing list
>Arab-gen from net.bio.net


-- 
       _      ____          __   ____  
      /  \   /          / \    /   \ \        Tobias I. Baskin
     /   /  /          /   \   \      \         Biology Department
    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
   /      /          /       \   \       \        University of Massachusetts
  /      /          /         \   \       \        Amherst, MA, 01003
/      / ___   /           \   \__/  \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243



------------------------------

Message: 2
Date: Wed, 17 Nov 2010 20:36:27 +0000
From: "Holt, Ben F. III" <benholt from ou.edu>
Subject: Re: [Arabidopsis] Petri Dish Racks
To: Arabidopsis Listserv <arab-gen from magpie.bio.indiana.edu>
Message-ID: <C90998EA.9C3E%benholt from ou.edu>
Content-Type: text/plain; charset="iso-8859-1"

I sent Sara a reply earlier, but just realized I didn't reply to the
listserv. Basically we use similar approaches to what Tobias described - my
reply to Sara is below.

Hi Sara,

We use three cheap methods and all work fine. First - the two super cheap,
probably don't have to buy anything options:
1) If you have some of those flats that are split up into multiple wells
(like 6X6 or something along those lines), you can flip it upside down and
wedge the plates in the cracks on the bottom - sounds pretty low rent, but
it works like a charm and only requires the effort of a flipping motion.
2) If you have an extra metal rack from the standard light shelving used in
most labs, just place it on top of your existing shelving and put the plates
in the cracks - this is my favorite option. We have one old piece of
shelving from who knows where and it holds many plates in our standard
growth room. [The baking supply store racks idea from Tobias sounds like a
great solution if you don't have any old shelving lying around]
3) Costs a few bucks option: If you are handy or have a handy person in your
department, just take a 4" tall piece of flat plexiglass sheet cut to your
preferred length (we have one that is 2.5-3 feet long) and find a few square
wood blocks that can be attached to the back (like a scrap piece from the
end of a 2"X4"). Cut one side of the block to get the desired angle of the
sheet once it is attached. For each block, drill a couple of holes through
the plexiglass and attach the block to the back of the sheet with wood
screws. To keep the plates sitting on the front side of the plexiglass sheet
you can epoxy a strip of plexiglass dowel at the bottom edge. If you don't
have any wood scraps, you can also cut a thin strip of plexiglass and use
heat to bend it to your desired angle and then epoxy 2-3 such strips to the
back of the flat sheet.

Ben

========
Ben Holt
Assistant Professor
University of Oklahoma
Department of Botany and Microbiology
GLCH Rm 219
770 Van Vleet Oval
Norman, OK  73019
Phone (405)325-9018
FAX   (405)325-7619
http://www.ou.edu/cas/botany-micro/faculty/holt.html




------------------------------

Message: 3
Date: Thu, 18 Nov 2010 08:06:01 +1100
From: Rosemary White <rosemary.white from csiro.au>
Subject: Re: [Arabidopsis] Petri Dish Racks
To: Tobias Baskin <baskin from bio.umass.edu>, SaraN
    <passiflora42 from yahoo.com>
Cc: "arab-gen from magpie.bio.indiana.edu"
    <arab-gen from magpie.bio.indiana.edu>
Message-ID: <C90A8EE9.1F4D7%rosemary.white from csiro.au>
Content-Type: text/plain; charset="US-ASCII"

And if you only need to hold a few plates vertically, another option is an
old-fashioned toast rack.  We source these from our local two dollar shop
(which is actually $USD2 now!)  You need to rummage around, petri dishes in
hand, to find one with the right spacing - for thick toast.
cheers,
Roseamry White


On 18/11/10 12:19 AM, "Tobias Baskin" <baskin from bio.umass.edu> wrote:

> Hi,
> We made our own by hand, lots of little strips of plexi
> glass. Its a pain. Its a bit easier to make them from wood, but still
> tedious. I have since learned that some baking cooling racks have
> spacings that are just right. You will have an amusing trip to the
> local supermarket or home-stuff store with your petri dish to find a
> product with optimal spacing. I have also seen plates placed all
> together in small boxes. This doesn't give space between each plate
> but if the boxes have shallow sides (like pipette tip boxes) it can
> be ok. Hope this helps.
> Tobias Baskin
> 
>> Does anyone know of a product that would hold petri dish racks
>> vertically?  We are growing Arabadopsis on plates in various solutions
>> and need them to be vertical so they grow upright.  Thanks in advance.
>> 
>> _______________________________________________
>> Arab-gen mailing list
>> Arab-gen from net.bio.net
> 




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