[Arabidopsis] vertical growth

Forde, Brian via arab-gen%40net.bio.net (by b.g.forde from lancaster.ac.uk)
Fri Nov 19 07:36:19 EST 2010


The method we have evolved over the years is rather low-tech, but
effective - and I think it has some advantages over other methods. 

If we are using round 90 mm Petri dishes (because they are much cheaper
than those square ones) we stack them together vertically, one against
the next in sets of up to 20 or so. To hold them in place we made some
simple wooden holders, the base of which is formed by a block of wood 40
cm in length x 9 cm wide x 1 cm thick onto the sides of which two strips
of wood 40 cm x  5 cm have been attached. This gives a holder that is
U-shaped in cross-section and into which the stacked petri dishes fit
nicely. (Materials other than wood, that are easier to wipe clean might
be preferable, although the block of wood does act as an insulator
against heat coming from lights on the shelf below, so this may help
prevent condensation on the lids). So that the plates at the two ends of
the stack don't experience different light conditions from the rest we
put a blank agar plate at each end. A simple piece of autoclave tape
placed across the top of the holder at each end of the stack can be used
as a bookend to stop plates toppling over.

This is an inexpensive and easy way to hold the plates and it doesn't
take up as much space as when you have a gap between each plate (in a
'toaster rack' type arrangement). The stacking also means that the roots
receive less light than they would otherwise (the holders are also
painted black) and I think there is less problem with condensation. 

Brian Forde

-----Original Message-----
From: arab-gen-bounces from oat.bio.indiana.edu
[mailto:arab-gen-bounces from oat.bio.indiana.edu] On Behalf Of Brian Cady
Sent: 18 November 2010 17:11
To: arab-gen from oat.bio.indiana.edu
Subject: [Arabidopsis] vertical growth

Here at Adan Colon-Carmona's lab we wrap bundles of 3-6 square plates
with a rubber band. When banded together, the unit will sit in the
incubator with each plate vertically aligned.

Brian Cady





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Today's Topics:

   1. Re: Petri Dish Racks (Tobias Baskin)
   2. Re: Petri Dish Racks (Holt, Ben F. III)
   3. Re: Petri Dish Racks (Rosemary White)


----------------------------------------------------------------------

Message: 1
Date: Wed, 17 Nov 2010 08:19:57 -0500
From: Tobias Baskin <baskin from bio.umass.edu>
Subject: Re: [Arabidopsis] Petri Dish Racks
To: SaraN <passiflora42 from yahoo.com>
Cc: arab-gen from magpie.bio.indiana.edu
Message-ID: <p06240504c90985ad185f@[10.0.1.2]>
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Hi,
    We made our own by hand, lots of little strips of plexi glass. Its a
pain. Its a bit easier to make them from wood, but still tedious. I have
since learned that some baking cooling racks have spacings that are just
right. You will have an amusing trip to the local supermarket or
home-stuff store with your petri dish to find a product with optimal
spacing. I have also seen plates placed all together in small boxes.
This doesn't give space between each plate but if the boxes have shallow
sides (like pipette tip boxes) it can be ok. Hope this helps.
            Tobias Baskin

>Does anyone know of a product that would hold petri dish racks 
>vertically?  We are growing Arabadopsis on plates in various solutions 
>and need them to be vertical so they grow upright.  Thanks in advance.
>
>_______________________________________________
>Arab-gen mailing list
>Arab-gen from net.bio.net


-- 
       _      ____          __   ____  
      /  \   /          / \    /   \ \        Tobias I. Baskin
     /   /  /          /   \   \      \         Biology Department
    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
   /      /          /       \   \       \        University of
Massachusetts
  /      /          /         \   \       \        Amherst, MA, 01003
/      / ___   /           \   \__/  \ ____
www.bio.umass.edu/biology/baskin
Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243



------------------------------

Message: 2
Date: Wed, 17 Nov 2010 20:36:27 +0000
From: "Holt, Ben F. III" <benholt from ou.edu>
Subject: Re: [Arabidopsis] Petri Dish Racks
To: Arabidopsis Listserv <arab-gen from magpie.bio.indiana.edu>
Message-ID: <C90998EA.9C3E%benholt from ou.edu>
Content-Type: text/plain; charset="iso-8859-1"

I sent Sara a reply earlier, but just realized I didn't reply to the
listserv. Basically we use similar approaches to what Tobias described -
my reply to Sara is below.

Hi Sara,

We use three cheap methods and all work fine. First - the two super
cheap, probably don't have to buy anything options:
1) If you have some of those flats that are split up into multiple wells
(like 6X6 or something along those lines), you can flip it upside down
and wedge the plates in the cracks on the bottom - sounds pretty low
rent, but it works like a charm and only requires the effort of a
flipping motion.
2) If you have an extra metal rack from the standard light shelving used
in most labs, just place it on top of your existing shelving and put the
plates in the cracks - this is my favorite option. We have one old piece
of shelving from who knows where and it holds many plates in our
standard growth room. [The baking supply store racks idea from Tobias
sounds like a great solution if you don't have any old shelving lying
around]
3) Costs a few bucks option: If you are handy or have a handy person in
your department, just take a 4" tall piece of flat plexiglass sheet cut
to your preferred length (we have one that is 2.5-3 feet long) and find
a few square wood blocks that can be attached to the back (like a scrap
piece from the end of a 2"X4"). Cut one side of the block to get the
desired angle of the sheet once it is attached. For each block, drill a
couple of holes through the plexiglass and attach the block to the back
of the sheet with wood screws. To keep the plates sitting on the front
side of the plexiglass sheet you can epoxy a strip of plexiglass dowel
at the bottom edge. If you don't have any wood scraps, you can also cut
a thin strip of plexiglass and use heat to bend it to your desired angle
and then epoxy 2-3 such strips to the back of the flat sheet.

Ben

========
Ben Holt
Assistant Professor
University of Oklahoma
Department of Botany and Microbiology
GLCH Rm 219
770 Van Vleet Oval
Norman, OK  73019
Phone (405)325-9018
FAX   (405)325-7619
http://www.ou.edu/cas/botany-micro/faculty/holt.html




------------------------------

Message: 3
Date: Thu, 18 Nov 2010 08:06:01 +1100
From: Rosemary White <rosemary.white from csiro.au>
Subject: Re: [Arabidopsis] Petri Dish Racks
To: Tobias Baskin <baskin from bio.umass.edu>, SaraN
    <passiflora42 from yahoo.com>
Cc: "arab-gen from magpie.bio.indiana.edu"
    <arab-gen from magpie.bio.indiana.edu>
Message-ID: <C90A8EE9.1F4D7%rosemary.white from csiro.au>
Content-Type: text/plain; charset="US-ASCII"

And if you only need to hold a few plates vertically, another option is
an old-fashioned toast rack.  We source these from our local two dollar
shop (which is actually $USD2 now!)  You need to rummage around, petri
dishes in hand, to find one with the right spacing - for thick toast.
cheers,
Roseamry White


On 18/11/10 12:19 AM, "Tobias Baskin" <baskin from bio.umass.edu> wrote:

> Hi,
> We made our own by hand, lots of little strips of plexi glass. Its a 
> pain. Its a bit easier to make them from wood, but still tedious. I 
> have since learned that some baking cooling racks have spacings that 
> are just right. You will have an amusing trip to the local supermarket

> or home-stuff store with your petri dish to find a product with 
> optimal spacing. I have also seen plates placed all together in small 
> boxes. This doesn't give space between each plate but if the boxes 
> have shallow sides (like pipette tip boxes) it can be ok. Hope this 
> helps.
> Tobias Baskin
> 
>> Does anyone know of a product that would hold petri dish racks 
>> vertically?  We are growing Arabadopsis on plates in various 
>> solutions and need them to be vertical so they grow upright.  Thanks
in advance.
>> 
>> _______________________________________________
>> Arab-gen mailing list
>> Arab-gen from net.bio.net
> 



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