[Arabidopsis] vertical growth

Grant R Cramer via arab-gen%40net.bio.net (by cramer from unr.edu)
Sat Nov 20 17:08:08 EST 2010


Hi John,
I think that Arabidopsis roots would be inhibited by light like other
roots. See http://www.plantphysiol.org/cgi/reprint/89/2/622.pdf for an
example. The horticultural industry has known about the inhibitory effects
of light on roots for many years. They generally use black pots for this
very reason. Colored pots will still let some light through inhibiting
root growth near the surface of the pot.

All the best,
Grant

Grant R. Cramer
Professor
Department of Biochemistry and Molecular Biology, MS 200
University of Nevada, Reno
Reno, NV 89557
(775) 784-4204
cramer from unr.edu
http://www.ag.unr.edu/cramer/





On 11/18/10 2:43 PM, "Turner John Prof (BIO)" <J.G.Turner from uea.ac.uk> wrote:

>Dear Brian and others posting on this,
>
>These are wonderfully inventive solutions, but I throw in a couple of
>cautions to think about.
>
>It is important that light intensity and light quality (spectrum) are
>uniform for the plants you grow: intensity is important for
>photosynthesis, but also light reflected from neighbouring seedlings
>alters the Red:FR ratio, and this does alter growth.
>
>Light on roots is generally "unnatural", but most of us grow plants in
>axenic culture which exposes roots to light, and I am told that this
>promotes development of proplastids in the roots.
>
>It would be really useful if someone can confirm that root growth in
>roots exposed to the light is not different from root growth in roots not
>exposed to the light.
>
>JOHN
>
>
>
>
>John Turner
>Professor of Plant Science
>Chair of the Centre for Contemporary Agriculture
>Norwich Research Park Professor for Biosciences
>Associate Dean for Enterprise and Engagement
>Honorary Faculty Member, John Innes Centre
>School of Biological Sciences
>University of East Anglia
>Norwich Research Park NR4 7TJ
>
>01603 592192
>07767668146
>
>http://www.contemporaryagriculture.com
>
>http://www.uea.ac.uk/bio/
>
>
>-----Original Message-----
>From: arab-gen-bounces from oat.bio.indiana.edu
>[mailto:arab-gen-bounces from oat.bio.indiana.edu] On Behalf Of Brian Cady
>Sent: 18 November 2010 17:11
>To: arab-gen from oat.bio.indiana.edu
>Subject: [Arabidopsis] vertical growth
>
>Here at Adan Colon-Carmona's lab we wrap bundles of 3-6 square plates
>with a 
>rubber band. When banded together, the unit will sit in the incubator
>with each 
>plate vertically aligned.
>
>Brian Cady
>
>
>
>
>
>________________________________
>From: "arab-gen-request from oat.bio.indiana.edu"
><arab-gen-request from oat.bio.indiana.edu>
>To: arab-gen from magpie.bio.indiana.edu
>Sent: Thu, November 18, 2010 12:03:51 PM
>Subject: Arab-gen Digest, Vol 67, Issue 7
>
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>Today's Topics:
>
>   1. Re: Petri Dish Racks (Tobias Baskin)
>   2. Re: Petri Dish Racks (Holt, Ben F. III)
>   3. Re: Petri Dish Racks (Rosemary White)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Wed, 17 Nov 2010 08:19:57 -0500
>From: Tobias Baskin <baskin from bio.umass.edu>
>Subject: Re: [Arabidopsis] Petri Dish Racks
>To: SaraN <passiflora42 from yahoo.com>
>Cc: arab-gen from magpie.bio.indiana.edu
>Message-ID: <p06240504c90985ad185f@[10.0.1.2]>
>Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
>Hi,
>    We made our own by hand, lots of little strips of plexi
>glass. Its a pain. Its a bit easier to make them from wood, but still
>tedious. I have since learned that some baking cooling racks have
>spacings that are just right. You will have an amusing trip to the
>local supermarket or home-stuff store with your petri dish to find a
>product with optimal spacing. I have also seen plates placed all
>together in small boxes. This doesn't give space between each plate
>but if the boxes have shallow sides (like pipette tip boxes) it can
>be ok. Hope this helps.
>            Tobias Baskin
>
>>Does anyone know of a product that would hold petri dish racks
>>vertically?  We are growing Arabadopsis on plates in various solutions
>>and need them to be vertical so they grow upright.  Thanks in advance.
>>
>>_______________________________________________
>>Arab-gen mailing list
>>Arab-gen from net.bio.net
>
>
>-- 
>       _      ____          __   ____
>      /  \   /          / \    /   \ \        Tobias I. Baskin
>     /   /  /          /   \   \      \         Biology Department
>    /_ /   __      /__ \   \       \__    611 N. Pleasant St.
>   /      /          /       \   \       \        University of
>Massachusetts
>  /      /          /         \   \       \        Amherst, MA, 01003
>/      / ___   /           \   \__/  \ ____
>www.bio.umass.edu/biology/baskin
>Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243
>
>
>
>------------------------------
>
>Message: 2
>Date: Wed, 17 Nov 2010 20:36:27 +0000
>From: "Holt, Ben F. III" <benholt from ou.edu>
>Subject: Re: [Arabidopsis] Petri Dish Racks
>To: Arabidopsis Listserv <arab-gen from magpie.bio.indiana.edu>
>Message-ID: <C90998EA.9C3E%benholt from ou.edu>
>Content-Type: text/plain; charset="iso-8859-1"
>
>I sent Sara a reply earlier, but just realized I didn't reply to the
>listserv. Basically we use similar approaches to what Tobias described -
>my
>reply to Sara is below.
>
>Hi Sara,
>
>We use three cheap methods and all work fine. First - the two super cheap,
>probably don't have to buy anything options:
>1) If you have some of those flats that are split up into multiple wells
>(like 6X6 or something along those lines), you can flip it upside down and
>wedge the plates in the cracks on the bottom - sounds pretty low rent, but
>it works like a charm and only requires the effort of a flipping motion.
>2) If you have an extra metal rack from the standard light shelving used
>in
>most labs, just place it on top of your existing shelving and put the
>plates
>in the cracks - this is my favorite option. We have one old piece of
>shelving from who knows where and it holds many plates in our standard
>growth room. [The baking supply store racks idea from Tobias sounds like a
>great solution if you don't have any old shelving lying around]
>3) Costs a few bucks option: If you are handy or have a handy person in
>your
>department, just take a 4" tall piece of flat plexiglass sheet cut to your
>preferred length (we have one that is 2.5-3 feet long) and find a few
>square
>wood blocks that can be attached to the back (like a scrap piece from the
>end of a 2"X4"). Cut one side of the block to get the desired angle of the
>sheet once it is attached. For each block, drill a couple of holes through
>the plexiglass and attach the block to the back of the sheet with wood
>screws. To keep the plates sitting on the front side of the plexiglass
>sheet
>you can epoxy a strip of plexiglass dowel at the bottom edge. If you don't
>have any wood scraps, you can also cut a thin strip of plexiglass and use
>heat to bend it to your desired angle and then epoxy 2-3 such strips to
>the
>back of the flat sheet.
>
>Ben
>
>========
>Ben Holt
>Assistant Professor
>University of Oklahoma
>Department of Botany and Microbiology
>GLCH Rm 219
>770 Van Vleet Oval
>Norman, OK  73019
>Phone (405)325-9018
>FAX   (405)325-7619
>http://www.ou.edu/cas/botany-micro/faculty/holt.html
>
>
>
>
>------------------------------
>
>Message: 3
>Date: Thu, 18 Nov 2010 08:06:01 +1100
>From: Rosemary White <rosemary.white from csiro.au>
>Subject: Re: [Arabidopsis] Petri Dish Racks
>To: Tobias Baskin <baskin from bio.umass.edu>, SaraN
>    <passiflora42 from yahoo.com>
>Cc: "arab-gen from magpie.bio.indiana.edu"
>    <arab-gen from magpie.bio.indiana.edu>
>Message-ID: <C90A8EE9.1F4D7%rosemary.white from csiro.au>
>Content-Type: text/plain; charset="US-ASCII"
>
>And if you only need to hold a few plates vertically, another option is an
>old-fashioned toast rack.  We source these from our local two dollar shop
>(which is actually $USD2 now!)  You need to rummage around, petri dishes
>in
>hand, to find one with the right spacing - for thick toast.
>cheers,
>Roseamry White
>
>
>On 18/11/10 12:19 AM, "Tobias Baskin" <baskin from bio.umass.edu> wrote:
>
>> Hi,
>> We made our own by hand, lots of little strips of plexi
>> glass. Its a pain. Its a bit easier to make them from wood, but still
>> tedious. I have since learned that some baking cooling racks have
>> spacings that are just right. You will have an amusing trip to the
>> local supermarket or home-stuff store with your petri dish to find a
>> product with optimal spacing. I have also seen plates placed all
>> together in small boxes. This doesn't give space between each plate
>> but if the boxes have shallow sides (like pipette tip boxes) it can
>> be ok. Hope this helps.
>> Tobias Baskin
>> 
>>> Does anyone know of a product that would hold petri dish racks
>>> vertically?  We are growing Arabadopsis on plates in various solutions
>>> and need them to be vertical so they grow upright.  Thanks in advance.
>>> 
>>> _______________________________________________
>>> Arab-gen mailing list
>>> Arab-gen from net.bio.net
>> 
>
>
>
>
>------------------------------
>
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