[Arabidopsis] Help with RNA Helicase Assay
(by asifashfaqk from gmail.com)
Wed Jun 1 15:40:56 EST 2011
We work with genes that are DEAD-BOX RNA Helicases and function to
negatively regulate response to multiple abiotic stress (Kant et.al. 2007).
In order to determine that these genes are functional RNA-helicases we have
cloned them in pET28a expression vector in frame with a HIS tag. We hope to
collaborate with a lab that does the RNA-dependent ATPase assay as well as
the actual RNA Helicase assay routinely in their lab.
Any kind of help is highly welcome.
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