[Arabidopsis] random hexamer vs oligo dT vs gene specific primer

Prakash M G via arab-gen%40net.bio.net (by pmgnair from gmail.com)
Thu Jun 2 19:49:39 EST 2011


Dear all,

May I know which one of the following method of cDNA synthesis is best for
doing real time PCR.

----- random hexamer vs oligo dT vs gene specific primer ------

Please give the advantage or reasons for following each method, particularly
in connection with real time PCR to check difference in trancription when
comparing a control set with an exposure group.



Thanks

Prakash


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