[Arabidopsis] Inquiry about making gentamicin agar plates

smith Chiang via arab-gen%40net.bio.net (by chiangscn from gmail.com)
Tue Jun 14 03:40:33 EST 2011


I has been using 1-3% sucrose in 1/2 xMS medium and I did observe
precipitation of Gem with Sigma Phytagel, but not with others
mentioned.



On Tue, Jun 14, 2011 at 1:37 PM, mintu desai <desai.185 from osu.edu> wrote:
> Why should you put 20% sucrose? Why? Arabidopsis seeds wild type or mutant
> would have enough carbon source and I wonder for what reason you want to put
> 20% sucrose? Gentamicin never precipitates in any usual recipe of 1X MS
> phytagar/Phytoblend or whatever agar plate.
> Thank you.
>
>
> On Sun, Jun 12, 2011 at 7:14 AM, smith Chiang <chiangscn from gmail.com> wrote:
>>
>> you should use other type of phytoagar, like that from Invitrogen
>> (Phytoagar) or Caissonlabs (Phytoblend). I also met such problem with
>> Sigma Phytagel a few years ago. I am using Phytoblend for many
>> antibioitic selections these years and it is pretty good.
>>
>> good luck!
>>
>>
>>
>>
>> On Fri, Jun 10, 2011 at 11:29 AM, Rashid Aman <rashidaman from hotmail.com>
>> wrote:
>> >
>> > Hi there..
>> >            I have some trouble with making gentamicin MS plates. I am
>> > using 1x MS, 20% sucrose, pH 5.7 and phtytagel for making the plates. But
>> > after autoclave when i cool down the bottle and add gentamicin, the
>> > gentamicin get precipitated. Could you please give me some suggestions to
>> > solve this problem.
>> >
>> >  Rashid aman
>> > PhD student Gyeongsang National UniversityJinju, South korea
>> >
>> >
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>



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