[Arabidopsis] RIKEN-FST lines and their footprints

[Joshua Mylne]

Dear Arabidopsis colleagues,

I am in the process of ordering a (RIKEN-FST) transposon-tagged line, but it appears they cannot confirm the insertion site using Ds-specific primers and primers for the supposed flanking gDNA sequence and so it seems the Ds element might have jumped out at some point in the seeds' amplification.

Does anyone have enough experience with these lines to know how often this Ds element leaves a footprint when they jump? I am trying to decide whether this line is likely still to be a mutant?

Thanks,

Josh

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Dr Joshua S. Mylne
ARC QEII Fellow

Chemistry & Structural Biology Division
Institute for Molecular Bioscience
The University of Queensland
St Lucia, QLD, 4072
AUSTRALIA

Office: +61 7 3346 2021
Lab Ph: +61 7 3346 2324
Fax No: +61 7 3346 2101
E-mail: j.mylne[at]imb.uq.edu.au

URL: www.researcherid.com/rid/A-5581-2009