[Arabidopsis] Methyl methanesulfonate instead of EMS?
(by j.mylne from imb.uq.edu.au)
Fri Oct 28 00:51:52 EST 2011
Has anyone tried using methyl methanesulfonate instead of EMS in classical Arabidopsis seed mutagenesis? If so, what did you find were the most common bases changes and also, what sort of dose did you find was an equivalent concentration to EMS?
There is plenty of evidence MMS is a suitable mutagen for Arabidopsis (e.g. Redei 1982 Mutation Research 99:243) and one important report that looked at point mutations in whole plants (Kovalchuk et al. 2000 EMBO J 19:4431) said MMS causes bases conversions and transversions, so creates a more diverse set of changes than EMS.
I would appreciate any comments about the use of MMS in Arabidopsis seed mutagenesis. I need to generate a more diverse set of amino acid changes than EMS as the typical G to A change has a limited spectrum of changes it can make and certain amino acids (e.g. Phe, Ile, Lys, Asn, Tyr) are immune.
Dr Joshua S. Mylne
ARC QEII Fellow
Chemistry & Structural Biology Division
Institute for Molecular Bioscience
The University of Queensland
St Lucia, QLD, 4072
Office: +61 7 3346 2021
Lab Ph: +61 7 3346 2324
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E-mail: j.mylne from imb.uq.edu.au
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