[Arabidopsis] question on SALK lines
(by ecker from salk.edu)
Sun Apr 1 18:58:03 EST 2012
PS how can you tell a "knock-down" by PCR?
Sent from my iPhone
On Apr 1, 2012, at 2:44 AM, Smith Chiang <chiangscn from gmail.com> wrote:
> Hello At researchers,
> In my lab, I found that not all SALK_xxxxxxC are homozygous knock-out
> lines, after extracting genomic DNA and runing PCR detection.
> some are not homozygous and some are not knock-out(knock-down
> instead). has anyone of you observed this?
> thanks for your reply.
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