[Arabidopsis] question on SALK lines

Joseph Ecker via arab-gen%40net.bio.net (by ecker from salk.edu)
Sun Apr 1 18:58:03 EST 2012


PS how can you tell a "knock-down" by PCR? 

Sent from my iPhone

On Apr 1, 2012, at 2:44 AM, Smith Chiang <chiangscn from gmail.com> wrote:

> Hello At researchers,
> 
> In my lab, I  found that not all SALK_xxxxxxC are homozygous knock-out
> lines, after extracting genomic DNA and runing PCR detection.
> some are not homozygous and some are not knock-out(knock-down
> instead). has anyone of you observed this?
> 
> thanks for your reply.
> 
> Smith
> 
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