[Arabidopsis] mRNA and sRNA from seedlings

Slotkin, R. Keith via arab-gen%40net.bio.net (by slotkin.2 from osu.edu)
Mon Feb 20 06:09:25 EST 2012


Hello,

My lab has noticed a very poor efficiency for isolating both mRNA and small RNA from some tissues, while other tissues seem fine. We are using Invitrogen's (now Life Technologies) Trizol product. As far as we can tell, there are abundant mRNA and sRNA before the isolation, but the Trizol seems to select for long non-mRNA. Invitrogen says the tissue shouldn't matter, but experimentally we see that it does. Can anyone suggest an RNA prep that can work from ANY tissue (seedling, seed, etc...), isolate all small RNAs (20-25nt), and recover a high percentage of the longer mRNAs?

Thank you,

R. Keith Slotkin
Assistant Professor
The Ohio State University

500 Aronoff Laboratory
318 West 12th Ave.
Columbus, OH, 43210

slotkin.2 from osu.edu<mailto:slotkin.2 from osu.edu>
https://molgen.osu.edu/people/slotkin




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