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[Arabidopsis] We have trouble in [35S]Methionine labeling

Satoshi Naito via arab-gen%40net.bio.net (by naito from abs.agr.hokudai.ac.jp)
Mon Jun 10 07:54:23 EST 2013

Dear all,

We have been doing [35S]methionine labeling in wheat germ extract in vitro translation. 

(1) Some months a go, it suddenly happened that labeling efficiency of Promega WGE/ [35S]Met from American Radiolabeled Chemicals became 10% or so of before.  

(2) We did parallel experiments using this new batch and more-than-half-decayed old batch with Promega and "home-made" WGE. The old batch worked much better for both Promega and home-made WGE.

(3) We then tried [35S]Met from Hungarian Acad Sci. It worked OK for that time, but the next batch did not work well.

(2) tells that something is wrong with  [35S]Met. But it should be rare that two different source have the same problem.

Do anyone out there experienced/experiencing similar trouble?

Satoshi Naito
Hokkaido University
Sapporo, Japan.

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