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[Arabidopsis] Re: Arab-gen Digest, Vol 102, Issue 14

Henrik Scheller via arab-gen%40net.bio.net (by hscheller from lbl.gov)
Wed Oct 30 12:52:20 EST 2013


Re:
Subject: [Arabidopsis] chloral hydrate for DIC microscopy

Because chloral hydrate is a restricted chemical in the US it is very hard
to get. It is also very hard to work with it since so many procedures need
to be followed to satisfy Drug Enforcement Agency and local regulations. We
gave up - it was too cumbersome. Supposedly, Visikol http://visikol.com/ is
a good alternative. We did not try it yet so it would be great if somebody
has experience with it.
Henrik Scheller



On Wed, Oct 30, 2013 at 10:03 AM, <arab-gen-request from oat.bio.indiana.edu>wrote:

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> Today's Topics:
>
>    1. chloral hydrate for DIC microscopy  (Shpak, Elena D)
>    2. Post-doctoral position available (manash.chatterjee from gmail.com)
>    3. Re: Inquiring about high background experienced with      BiFC in
>       protoplasts (Harley)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 28 Oct 2013 18:01:30 +0000
> From: "Shpak, Elena D" <eshpak from utk.edu>
> Subject: [Arabidopsis] chloral hydrate for DIC microscopy
> To: "arab-gen from net.bio.net" <arab-gen from magpie.bio.indiana.edu>
> Message-ID:
>         <FC9BB3C01993A44F89D793ADA067549864F53808 from kmbx4.utk.tennessee.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> My lab uses chloral hydrate for DIC microscopy of Arabidopsis tissues.
> Originally we bought it from Fisher but they do not carry it anymore. Most
> recently we bought chloral hydrate from Spectrum Chemicals but now it is on
> a backorder indefinitely and the vendor does not have any date that it
> would be available. A year and a half ago we bought chloral hydrate from
> Sigma. We got two different batches but in both cases pH was so low (0.5)
> that it would destroy Arabidopsis tissues almost immediately. Sigma
> reimbursed us however we had to switch to Spectrum Chemicals. Now Sigma has
> a backorder on chloral hydrate until February. Have anybody got chloral
> hydrate from them recently? Is the quality of it got any better? If not is
> it possible to pH that chloral hydrate and make it usable? (We tried but
> were not successful.) Is there any other source for chloral hydrate in the
> USA?
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 28 Oct 2013 04:43:33 -0700 (PDT)
> From: manash.chatterjee from gmail.com
> Subject: [Arabidopsis] Post-doctoral position available
> To: bionet-genome-arabidopsis from moderators.isc.org
> Message-ID: <f482eb0d-e9e4-45be-ad5f-7dc640716252 from googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> POST-DOC position
>
> The URGV (Unité de recherche en génomique végétale), implanted in Génopole
> of Evry, is a laboratory supported by INRA to pursue research on various
> aspects of plant genomics. BenchBio is a plant biotech company located in
> Vapi city, Gujarat India that focus on engineering new plant prototypes
> with novel traits
>
> We are recruiting a scientist at the post-doctoral level to work on a
> joint project to improve melon plants by TILLING funded by CEFIPRA (
> http://www.cefipra.org). Further details will be provided to the
> interested candidates.  The candidates will benefit from automated platform
> of positional cloning, gene knockout, TILLING etc at URGV lab. This will
> allow them to have a good experience in the state of the art technologies
> in plant genomics
>
> Candidate must have a PhD in the areas of Molecular Biology, Genetics,
> Genomics or bioinformatics and speak English and/or French
>
> Location: URGV-INRA, 2 Rue Gaston Crémieux, 91057, Evry Cedex, France
> Starting date: as soon as possible
> Duration: Two years
>
> Contacts:
> Dr Abdel Bendahmane, URGV-INRA, 2 Rue Gaston Crémieux, 91057, Evry Cedex,
> France. bendahm from evry.inra.fr, tel: 0033160874502; Fax0033160874510
>
> Dr Manash Chatterjee, BenchBio Pvt Ltd.,c/o Jai Research Foundation (JRF),
> Vapi Gujarat, India, http://www.benchbio.com, email: info from benchbio.com
>
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 29 Oct 2013 14:05:17 -0700 (PDT)
> From: Harley <harleyk from gmail.com>
> Subject: [Arabidopsis] Re: Inquiring about high background experienced
>         with    BiFC in protoplasts
> To: bionet-genome-arabidopsis from moderators.isc.org
> Message-ID: <4188b712-66c4-432b-99d2-ad7c3e173e1c from googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Richard, I don't have much experience with BiFC in protoplasts, but I do a
> lot of BiFC in Nicotiana benthamiana. Have you tested your constructs in
> this plant?
>
>
>
> ------------------------------
>
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> End of Arab-gen Digest, Vol 102, Issue 14
> *****************************************
>



-- 
Henrik Vibe Scheller
Joint BioEnergy Institute, Feedstocks Division
Lawrence Berkeley National Laboratory
Adjunct Professor, UC Berkeley, Dept. of Plant and Microbial Biology
5885 Hollis St., 4th floor
Emeryville, CA 94608

Tel.: 510-486-7371 (office)
       510-207-0283 (cell)
Fax:510-486-4252


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