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[Arabidopsis] CaMV35S min promoter

Bernd Weisshaar via arab-gen%40net.bio.net (by weisshaa from cebitec.uni-bielefeld.de)
Mon Jun 8 02:52:15 EST 2015


this is a trip back into history.

You can have a look here:


The vector that might be most helpful is pBT10-GUS
The sequence is linked as a text file in the (very old) CGC format.
Together with the map you should be able to find the sequence you want.

Note that we used (and still use) the -46 to +8 version of the minimal 35S promoter. There is also a -90 version. Some background info is in this old paper:
(Recently, SEB allowed to make this old paper publicly available as a PDF which is linked at the URL above.). 

Bests, Bernd

> On 08.06.2015, at 02:27, Smith Chiang <chiangscn from gmail.com> wrote:
> Hello everyone,
> could anyone tell me the sequence of CaMV35S minimal promoter? I
> searched for a while but failed to know it. I need to know the
> sequence so as to design primers for constructing a vector.
> thanks,
> Smith
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Bielefeld University, CeBiTec & Department of Biology
Prof. Dr. Bernd Weisshaar
Chair of Genome Research / Center for Biotechnology
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33615 Bielefeld

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