[Arabidopsis] CaMV35S min promoter
(by weisshaa from cebitec.uni-bielefeld.de)
Mon Jun 8 02:52:15 EST 2015
this is a trip back into history.
You can have a look here:
The vector that might be most helpful is pBT10-GUS
The sequence is linked as a text file in the (very old) CGC format.
Together with the map you should be able to find the sequence you want.
Note that we used (and still use) the -46 to +8 version of the minimal 35S promoter. There is also a -90 version. Some background info is in this old paper:
(Recently, SEB allowed to make this old paper publicly available as a PDF which is linked at the URL above.).
> On 08.06.2015, at 02:27, Smith Chiang <chiangscn from gmail.com> wrote:
> Hello everyone,
> could anyone tell me the sequence of CaMV35S minimal promoter? I
> searched for a while but failed to know it. I need to know the
> sequence so as to design primers for constructing a vector.
> Arab-gen mailing list
> Arab-gen from net.bio.net
Bielefeld University, CeBiTec & Department of Biology
Prof. Dr. Bernd Weisshaar
Chair of Genome Research / Center for Biotechnology
genomforschung from uni-bielefeld.de (Secretary)
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