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[Arabidopsis] Re: Similar phenotype in overexpression and knockout

Charles S Gasser via arab-gen%40net.bio.net (by csgasser from ucdavis.edu)
Thu Apr 5 13:44:46 EST 2018

We found similar phenotypic effects on ovule development from over/ectopic expression and loss of function for Class III HDzip genes.  We believe that these genes act in opposition to other genes and disruption of a balance in either direction leads to similar phenotypic effects.  We published this explanation:

Kelley, D. R., Skinner, D. J., and Gasser, C. S.  (2009) Roles of polarity determinants in ovule development. Plant J. 57: 1054-1064.
doi: 10.1111/j.1365-313X.2008.03752.x 
 Hope this is useful.  At least you could reference that such a thing has been previously observed.

Chuck Gasser

在 2010年5月3日星期一 UTC-3上午9:01:31,Ido Kron写道:
> I am puzzled that both over-expression and knockout of the kinase that I am
> working on result in the same phenotype (a reduction in the expression of
> several putative target genes, compaired with wild-type plants.) Does anyone
> know of other cases when over-expression and knockout mutations of a gene
> result in similar phenotypes.
> Thank you very much,
> Ido Kron
> The Hebrew University of Jerusalem

Hello Dr. Kron,

I am faced with the same situation as you posted here. May you tell me how you interpret your data finally?

Thanks in millions,

Charles S. Gasser
Professor Emeritus
Dept. of Molecular and Cellular Biology
University of California, Davis
1 Shields Ave.
Davis, CA  95616

104 Briggs Hall
530 752-1013

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