raw 2 channel image data available?

Simon Andrews simon.andrews at bbsrc.ac.uk
Wed Aug 29 06:17:48 EST 2001


Chris Bowman wrote:
> 
> I would like to try my hand at image processing and analysis on
> microarray images, but I'm having a bit of a problem getting some
> sample data.  Everything I've found so far at most of the standard
> databases are 8 bit .gif images, which aren't really very suitable for
> developing new algorithms for spot/background intensity estimates,
> because its difficult to accurately seperate the 2 dye colors.
> Ideally I'd like to be able to work on the original pair of 16 (or 8
> even) bit .tiff images that come directly from the scanners, but I
> haven't been able to find samples of such images online.  Anyone know
> if I can get these somewhere?

You could try sending off for the free demos for some of the commercial
products which do this.  They usually include some sample images.  This
would also allow you to compare your results against those of the
existing programs.

I know that the ImaGene demo has several example arrays on it.  You can
get it (after registration) from;

	http://www.biodiscovery.com/products/ImaGene/downloads.html

I would imagine that a search on Google will turn up several more
programs like this.

Alternatively, you could try a literature search for groups near you who
are doing microarray analysis, and ask them for some data.  Seeing as
you don't need to know what spots are on the array, they shouldn't have
a problem supplying you with some data.

	Hope this helps.

	Simon.





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