New Gene Quantification Homepage and FREE Software !!!

genequantification gene.quantification at wzw.tum.de
Tue Apr 2 06:46:57 EST 2002


http://www.wzw.tum.de/gene-quantification/

Main focus of GENE QUANTIFICATION web page is to describe 
and summarize all technical aspects involved in quantitative  
gene expression analysis using real-time (RT-) PCR. 
( specificity, sensitivity, reproducibility, intra- and inter-assay
variations, etc.)

The GENE QUANTIFICATION web page illustrates  
the usefulness of a reliable quantification strategy,  
and the difference between absolute vs. relative quantification  
assays in real-time (RT-) PCR. 

RT-PCR is the technique of choice for analysing mRNA in extremely low
abundance.
Real-time RT-PCR using SYBR Green I detection combines the ease  
and necessary exactness to be able to produce reliable as well as
rapid results.
To obtain high accuracy and reliability in RT and real-time PCR highly
defined
quantification strategies and methods are needed.

http://www.wzw.tum.de/gene-quantification/  content:

- Real-time (RT-) PCR  and  optimisation !!! 
- Available  real-time PCR  platforms 
- Description  of  quantification  strategies  in  real-time (RT-) PCR
- Description  of  absolute quantification  and  relative
quantification
- REST © ( Relative  Expression  Software  Tool © ) 
- Housekeeping Gene  problematic  in  real-time PCR  ( under
construction !! )
- Verification of array results via kinetic RT-PCR  ( under
construction !! )
- Comparison  of   real-time RT-PCR   versus   competitive RT-PCR 
- Download  of  software  tools  (e.g.  REST © ) 
- Summary  of  relevant  literature 
- Summary  of  relevant  links 


for further information contact Dr. Michael W. Pfaffl at 

http://www.wzw.tum.de/gene-quantification/





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