Gene Quantification Newsletter 13. August 2002
NEW features on http://www.wzw.tum.de/gene-quantification/
Endocrinology - NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/endo.html
NEW ENDOCRINOLOGY POSTERS on Poster Boards
http://www.wzw.tum.de/gene-quantification/poster-a.html
Housekeeping Gene & Normalisation in kinetic PCR
http://www.wzw.tum.de/gene-quantification/hkg.html
real-time PCR and RT-PCR - NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/real-time.html
and external links:
Q-Gene Software
Processing of gene expression data generated by quantitative real-time RT-PCR
geNORM
TATAA Biocenter: q-PCR Network of Excellence
NEW real-time PCR primer and probe Databases
Main focus of GENE QUANTIFICATION web page is to describe
and summarize all technical aspects involved in quantitative
gene expression analysis using real-time PCR & RT-PCR.
( specificity, sensitivity, reproducibility, intra- & inter-assay variations,
kinetic PCR efficiency calculation, optimisation strategy, etc.)
The GENE QUANTIFICATION web page illustrates the usefulness of a reliable
quantification strategy, and the difference between absolute vs. relative
quantification
assays in kinetic PCR & kinetic RT-PCR.
RT-PCR is the technique of choice for analysing mRNA in extremely low
abundance.
Real-time RT-PCR using SYBR Green I detection combines the ease and necessary
exactness to be able to produce reliable as well as rapid results.
To obtain high accuracy and reliability in RT and real-time PCR highly defined
quantification strategies and calculation methods are needed.
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Gene Quantification @ wzw.tum.de
e-mail: gene.quantification at wzw.tum.de
homepage: http://www.wzw.tum.de/gene-quantification/
responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY
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