ratio of AA-dUTP and dTTP

Niel Karrow nkarrow at ovc.uoguelph.ca
Thu Jan 24 15:10:46 EST 2002


I was wondering if anyone could please explain why one has to
incorporate dTTP into the master mix with AA-dUTP? Is there an optimum
ratio? It would seem to me that one could perform the RT without any
dTTP and get a stronger flourescent signal from Cy3/Cy5 because more
AA-dUTP was incorporated into the product. Lastly, if no dTTP was used,
would the product not be cRNA rather than cDNA? Thanks in advance,
Niel
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