determining specific activity

Hip Tripper cbgbzzzz at hotmail.com
Wed Mar 6 12:43:45 EST 2002


How does one determine the specific activity of a labeled sample? 
Does anyone do this?

I did an amino-allyl labeling and the resulting conjugation to
Amersham's mono-functional yielded what subjectively looked great
(very dark purple following multiple washes).  However the S/N was
very low and I was not able to get any data from the hyb.  I would
like a means of assessing the sample prior to hyb'ing.

Thanks in advance!





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