determining specific activity
cbgbzzzz at hotmail.com
Wed Mar 6 12:43:45 EST 2002
How does one determine the specific activity of a labeled sample?
Does anyone do this?
I did an amino-allyl labeling and the resulting conjugation to
Amersham's mono-functional yielded what subjectively looked great
(very dark purple following multiple washes). However the S/N was
very low and I was not able to get any data from the hyb. I would
like a means of assessing the sample prior to hyb'ing.
Thanks in advance!
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