Gene quantification mailing 23. May 2002

[Dr. Michael Pfaffl]

Gene quantification mailing 23. May 2002

http://www.wzw.tum.de/gene-quantification/  NEW features
·       RT-PCR applications  in  Endocrinology
·       RT-PCR applications  in  Mammary Gland Immunology
·       Methodological  &  physiological  Poster Boards
·       Verification of array results
·       PCR, RPA, Northern-Blot & Southern-Blot movies
·       New versions of REST © and REST-XL ©


http://www.wzw.tum.de/gene-quantification/  content:
1.      real-time  PCR  and  RT-PCR
2.      mRNA structure  and  overview of mRNA analysis methods
3.      Optimization strategies for kinetic RT-PCR
4.      Available  real-time PCR  platforms
5.      Classical   block RT-PCR
6.      PCR, RPA, Northern-Blot & Southern-Blot movies
7.      Quantification  strategies  in  real-time RT-PCR
8.      Description  of  absolute quantification  and  relative quantification
9.      Relative  Expression  Software  Tool  -  REST ©
10.     Download  of  software  tools  (REST © and REST-XL ©)
11.     Housekeeping Gene  problematic  ( still under construction !!! )
12.     Verification of array results
13.     Comparison  of   real-time RT-PCR   vs.   competitive RT-PCR
14.     Summary  of  relevant  Literature
15.     Methodological  &  physiological  Poster Boards
16.     Summary  of  relevant  links
17.     Newsgroups  &  Biotech Boards

http://www.wzw.tum.de/gene-quantification/

Main focus of GENE QUANTIFICATION web page is to describe
and summarize all technical aspects involved in quantitative
gene expression analysis using real-time PCR  &  RT-PCR.
( specificity, sensitivity, reproducibility, intra- & inter-assay variations,
kinetic PCR efficiency calculation, optimisation strategy, etc.)

The GENE QUANTIFICATION web page illustrates the usefulness of a reliable
quantification strategy, and the difference between absolute vs. relative 
quantification
assays in kinetic PCR & kinetic RT-PCR.

RT-PCR is the technique of choice for analysing mRNA in extremely low 
abundance.
Real-time RT-PCR using SYBR Green I detection combines the ease and necessary
exactness to be able to produce reliable as well as rapid results.
To obtain high accuracy and reliability in RT and real-time PCR highly defined
quantification strategies and calculation methods are needed.

For further questions contact Dr. Michael W. Pfaffl at
gene.quantification at wzw.tum.de



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Gene Quantification @ wzw.tum.de

e-mail:         gene.quantification at wzw.tum.de
homepage:               http://www.wzw.tum.de/gene-quantification/

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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