Gene Quantification Newsletter - 09/2002

[Gene Quantification Homepage]

Gene Quantification Newsletter
September 2002

Main focus of the GENE QUANTIFICATION web page 
(http://www.wzw.tum.de/gene-quantification/) is, to describe and summarize 
all technical aspects involved in quantitative gene expression analysis 
using real-time (kinetic) PCR  &  RT-PCR.
e.g. detection chemistry, assay specificity, sensitivity, reproducibility, 
robustness, intra- & inter-assay variations, kinetic PCR efficiency 
calculation, quantification strategies, optimisation strategy, data 
calculation and statistics,  etc. etc.

The GENE QUANTIFICATION web page illustrates the usefulness of reliable 
quantification strategies, e.g. absolute-, relative-quantification assays, 
and the difference between them in kinetic PCR & kinetic RT-PCR.

Main focus of the PHYSIOLOGY web page is, to describe all kind of kinetic 
RT-PCR applications in molecular Physiology, molecular Endocrinology, and 
molecular Immunology (mainly mammary gland immunology) are summarized on my 
new homepage   http://www.wzw.tum.de/gene-quantification/physiology.shtml

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NEW features on
http://www.wzw.tum.de/gene-quantification/
&
http://www.wzw.tum.de/gene-quantification/physiology.shtml
& a new PASSWORD protected section
http://www.wzw.tum.de/gene-quantification/intranet/
(The INTRANET is only accessible for members of the Institute of Physiology)

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Endocrinology & Immunology  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/physiology.shtml

Determination of real-time PCR efficiency  -  NEW SECTION
http://www.wzw.tum.de/gene-quantification/efficiency.html

A lot of interesting NEW MOVIES
http://www.wzw.tum.de/gene-quantification/movie.html
e.g.
PCR, RPA, Electrophoresis, Northern- & Southern-Blot

New poster board organisation
http://www.wzw.tum.de/gene-quantification/poster-a.html

Kinetic PCR fluorescence detection chemistry
http://www.wzw.tum.de/gene-quantification/chemistry.html

Housekeeping Gene & Normalisation in kinetic PCR
http://www.wzw.tum.de/gene-quantification/hkg.html

real-time  PCR  and  RT-PCR  -  NEW PUBLICATIONS
http://www.wzw.tum.de/gene-quantification/real-time.html
and
http://www.wzw.tum.de/gene-quantification/literature.html
e.g.
Quantification of mRNA using real-time reverse transcription PCR: trends 
and problems by  Bustin SA. J Mol Endocrinol. 2002 29: 23-29
Absolute quantification of mRNA using real-time reverse transcription PCR 
assays by  Bustin SA   Journal of Molecular Endocrinology 25: 169-193 ( 2000)

LightCycler University (LCU)
LightCycler University (LCU) is a place where all levels of education can 
come to gain new insights, brush up on their skills or share ideas on using 
the LightCycler Instrument for researching DNA and other areas.
http://www.wzw.tum.de/gene-quantification/real-time.html#lcu

New links & databases:
http://www.realtimeprimerdatabase.ht.st
http://www.realtimeprimers.org/
http://www.pcrlinks.com


Please send the NEWSLETTER and the home page links to further scientists 
and friends who are interested in relative or absolute gene quantification 
using kinetic PCR.

best regards

Michael Pfaffl

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For further questions concerning any kind of real-time PCR and if you want 
to be included to our monthly appearing Gene Quantification Newsletter 
please contact gene.quantification at wzw.tum.de   You will receive all NEW 
FEATURES and future REST &  REST-XL updates.

"Gene Quantification" web page is a non-commercial, educational site with 
the only purpose of facilitating access to biology-related information over 
the internet. The web page is optimised for Netscape Navigator version 4.7 
& Internet Explorer version 5.0 (and higher versions) with an optimal 
screen resolution of 1024 x 768 pixel.

If you want to be removed from all future NEWSLETTERS please contact 
gene.quantification at wzw.tum.de. You will be removed instantly.


********************************************************************
Gene Quantification @ wzw.tum.de

e-mail: gene.quantification at wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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