Dear all,
At the outset this mail finds you all in good moods.
I want some guidance regarding my research work.
I have cytokine genes (MIP 1 alpha,CXC chemokine and
IL 1 beta of Japanese flounder fish)cloned in Pci neo
expression vector and also coat protein gene of
Nervous necrosis virus gene cloned in the same vector.
I would like to test the adjuvant effects of cytokine
genes in recombinant DNA vaccine using coat protein
gene of Nervous necrosis virus.I want to use DNA
microarrays and real time PCR to do this work.I am not
getting an idea how to plan this experiment.It will be
of great help If any one can give me the guidance how
to test the adjuvant effects by microarrys and real
time pcr.
with best regards,
Dhanwanthari.
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