Gene Quantification Newsletter - March 2003

[Gene Quantification Homepage]

Gene Quantification Newsletter  March 2003


New features:

2nd Nucleic Acid Quantification Meeting  in  London, UK
http://www.mds.qmw.ac.uk/surgery/events/Announcement.html

Amplifications for real-time qPCR Analysis workshop
https://dynamic.mjr.com/workshop/home.jsp

Quantitative real-time PCR course at RoboScreen
http://www.roboscreen.com/home/frame.htm


Gene Quantification in Endocrinology & Immunology  -  NEW PUBLICATIONS

Determination of most stable housekeeping genes, differentially regulated 
target genes and sample integrity: BestKeeper© - Excel© spreadsheet tool 
using a Repeated Pair-wise Correlation and Regression Analysis
21. Dechema Jahrestagung der Biotechnologen 2.-4. April 2003
http://www.wzw.tum.de/gene-quantification/poster-f.html

Tissue-specific expression pattern of bovine prion gene 
(PrP):  Quantification using real-time RT-PCR
http://www.wzw.tum.de/gene-quantification/absolute.html#prp

Detection and quantification of mRNA-expression of alpha- and beta-adrenergic
receptor subtypes in the mammary gland of dairy cows.
http://www.wzw.tum.de/gene-quantification/endo.html#adrenerg

The gastrointestinal tract as target of steroid hormone action: 
Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta 
and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR
http://www.wzw.tum.de/gene-quantification/endo.html#erab

Characterisation of gene expression patterns in 22RV1 cells for 
determination of environmental androgenic / antiandrogenic compounds
http://www.wzw.tum.de/gene-quantification/endo.html#compounds

Gene Expression of Immunologically Important Factors in Blood Cells, Milk 
Cells and Mammary Tissue of Cows.
http://www.wzw.tum.de/gene-quantification/immuno.html#papers

Insulin-like growth factor and insulin receptors in intestinal mucosa of 
neonatal calves.
http://www.wzw.tum.de/gene-quantification/endo.html#igfs


New Section BioInformatics in real-time PCR

http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml

BestKeeper© software - Determination of most stable housekeeping genes, 
differentially regulated target genes and template 
integrity:  BestKeeper©    Excel © spreadsheet tool using a Multiple 
Correlation and Regression Analysis
http://www.wzw.tum.de/gene-quantification/bestkeeper.html

LinRegPCR  -  Assumption-free analysis of quantitative real-time PCR data
http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-4

BioInformatics  -  Poster board
http://www.wzw.tum.de/gene-quantification/poster-f.html

Determination of real-time PCR efficiency
http://www.wzw.tum.de/gene-quantification/efficiency.html

NEW EQUATIONS & ALGORITHM by Tichopad et al. 2002
http://www.wzw.tum.de/gene-quantification/efficiency.html#e-fluoro-sig-2


NEW PUBLICATIONS about kinetic PCR
http://www.wzw.tum.de/gene-quantification/real-time.html
and
http://www.wzw.tum.de/gene-quantification/literature.html


Please send the NEWSLETTER and the home page links to further scientists 
and friends who are interested in real-time PCR, new quantification 
strategies, relative or absolute gene quantification using kinetic PCR.

best regards

Michael Pfaffl

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For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE 
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future REST &  REST-XL  &  BestKeeper   updates.

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********************************************************************
Gene Quantification @ wzw.tum.de

e-mail: gene.quantification at wzw.tum.de
homepage:       http://www.wzw.tum.de/gene-quantification/index.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/physiology.shtml
homepage:       http://www.wzw.tum.de/gene-quantification/bioinformatics.shtml

responsible Senior Scientist:
Dr. Michael W. Pfaffl
Institute of Physiology
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan
GERMANY

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