small amount labelling

Evelyn emwesseling at hotmail.com
Fri May 14 03:14:44 EST 2004


Hello,

I'm hoping that somebody can give me some advice about amino-allyl
labelling for small amount of amplified aRNA.
We want to amplified totRNA of single cell in 2 amplification rounds.
To make sure the protocols are working we are using 10 ng totRNA as a
test. The amount of amplified aRNA we find after 2 amplification
rounds is just enough for the labelling protocol I'm using now.
But when we want to use single cell the amplified aRNA is propably not
enough for the labelling protocol.
Does anybody have a any experiments with labelling amplified single
cell aRNA?

Greetings, 
Evelyn





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