[Arrays] 1-colour vs. 2-colour arrays?

ahmed essaghir ahmed.essaghir at gmail.com
Wed Jan 25 03:38:13 EST 2006


hi!
here is the link to Dan Nettleton's (Iowa State University) workshop, it
contains slides with basic exlpanations for both two-color and affymetrix
micraorrays from the technology they use till deep statistical analysis
(normalizing, tmulti-tests, clustering, ...).
http://www.public.iastate.edu/~dnett/MicroShortCourse/workshop.shtml
have fun!


On 1/24/06, arrays-request at oat.bio.indiana.edu <
arrays-request at oat.bio.indiana.edu> wrote:
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>    1. 1-colour vs. 2-colour arrays? (Jose McNach)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: 23 Jan 2006 10:34:26 -0800
> From: "Jose McNach" <josenet at tiscali.co.uk>
> Subject: [Arrays] 1-colour vs. 2-colour arrays?
> To: bionet-molbio-genearrays at moderators.isc.org
> Message-ID: <1138041266.280407.24190 at z14g2000cwz.googlegroups.com>
> Content-Type: text/plain; charset="iso-8859-1"
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>
> I've been using cDNA spotted arrays for a little while, and now we're
> at a point where we'd like to make use of certain arrays provided by
> Nimblegen, which are essentially Affymetrix style (only created with a
> slightly different technology) and I'm trying to familiarise myself
> with the different way to normalise and analyse data in this system...
>
> This prompted the question to me... actually two questions:
>
> 1) what would make someone choose one or another method?
>
> 2) why doesn't anyone use two colour hybs on Affymetrix style arrays?
>
>
> 1-colour systems seem efficient when one wants to compare a reference
> or two with many different samples. Also one avoids the dye-bias
> issue... Is there anything else important that I am missing? why not
> use 2-colour hybs also on Affymetrix type chips? are all the Affymetrix
> scanners 1-laser only?
>
> I've been doing a little google search but I don't seem to find
> conclusive answers...
>
> Jose
>
>
>
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