[Arrays] qPCR NEWS March 2009 - focus on HRM & HRM dyes

Editor www.Gene-Quantification.info via arrays%40net.bio.net (by editor from gene-quantification.info)
Thu Mar 26 05:30:14 EST 2009

qPCR NEWS March 2009 - focus on HRM & HRM dyes

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- HRM page updated with new papers
- NEW page - HRM dyes
- Online translation service of the Gene Quantification
- New qPCR events in autumn 2009
- New qPCR workshop modules at the TATAA Biocenter Germany
- Online translation service of the Gene Quantification =>

New qPCR workshop modules at the TATAA Biocenter Germany =>
European wide qPCR application workshops =>  register now !
=> course program spring - summer 2009
=> http://www.gene-quantification.de/bioeps-courses-spring-summer-2009.pdf


High Resolution Melting (HRM) is a novel, homogeneous, close-tube,
post-PCR method, enabling genomic researchers to analyze genetic
variations (SNPs, mutations, methylations) in PCR amplicons. It goes
beyond the power of classical melting curve analysis by allowing to
study the thermal denaturation of a double-stranded DNA in much more
detail and with much higher information yield than ever before. HRM
characterizes nucleic acid samples based on their disassociation
(melting) behavior. Samples can be discriminated according to their
sequence, length, GC content or strand complementarity. Even single
base changes such as SNPs (single nucleotide polymorphisms) can be
readily identified.


The most important High Resolution Melting application is gene
scanning - the search for the presence of unknown variations in PCR
amplicons prior to or as an alternative to sequencing. Mutations in
PCR products are detectable by High Resolution Melting because they
change the shape of DNA melting curves. A combination of new-
generation DNA dyes, high-end instrumentation and sophisticated
analysis software allows to detect these changes and to derive
information about the underlying sequence constellation.

HRM Applications => new papers
The introduction of HRM has renewed interest in the utility of DNA
melting for a wide range of uses, including:
Mutation discovery (gene scanning)
Screening for loss of heterozygosity
DNA fingerprinting
SNP genotyping
Characterization of haplotype blocks
DNA methylation analysis
DNA mapping
 Species identification
Somatic acquired mutation ratios
HLA compatibility typing
Association (case/control) studies
Allelic prevalence in a population
Identification of candidate predisposition genes



High Resolution Melting Dyes & probes


The secret of High Resolution Meling (HRM) analysis is to monitor this
process happening in real-time. This is achieved by using saturating
fluorescent dye. HRM dyes are known as intercalating dyes bind
specifically and in high amount (= saturating dye, except SYBR Green)
to double-stranded DNA and when they are bound they fluoresce
brightly. In the absence of double-stranded DNA they have nothing to
bind to and they only fluoresce at a low level (estimations are
between 1-4% false positive fluorescence). At the beginning of the HRM
analysis, after complete PCR amplification, there is a high level of
fluorescence in the sample because of the millions of copies of the
amplicon. But as the sample DNA is heated up and the two strands of
the DNA denaturate there is no longer any double stranded DNA present
and thus fluorescence is reduced => http://hrm-dyes.gene-quantification.info/

*  LC Green   * SYTO9   * Eva Green   * BEBO *   SYBR Green

online translation

Since October 2008 we provide an online translation service of the
Gene Quantification pages to several languages. Please recognize this
is an automatic and robotic based translation service, and therefore
we can give NO guarantee about the generated content. It should help
to understand the "rough" content of the Gene Quantification pages,
but still the original is the ENGLISH version:



With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
=>  Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory



Upcoming Events World-wide academic and commercial qPCR Events

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, etc.
Please submit your qPCR event here  =>  events from gene-



BioEPS GmbH / TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a
3-day qPCR Core Module and a 2-day qPCR Biostatistics Module.  All
courses are held regularly in Göteborg, Sweden, in English and in
Freising-Weihenstephan, Germany, in German and English, and in Prague,
Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide.
TATAA Biocenter Germany workshops are held in cooperation with BioEPS
GmbH, located at the campus of the Technical University of Munich, in
Freising-Weihenstephan, very close to the Munich Airport (MUC). For
more information and registration, please see our web page:
=> http://TATAA.gene-quantification.info/

Course Occasions 2009:

3-day qPCR Core Module  (Mon. - Wed.)
2-day BioStatistics Module (Thu. - Fri.)
3-day single-cell qPCR Module  (Mon. - Wed.)
3-day microRNA Module   (Mon. - Wed.)

20 - 22 April 2009  (E)      NEW microRNA qPCR

11 - 15 May 2009 (Deutsch)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module
(Thu. - Fri.)

15 - 19 Juni 2009  (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module
(Thu. - Fri.)

13 - 15 Juli 2009  (E)         NEW microRNA qPCR

27 - 31 Juli 2009  (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module
(Thu. - Fri.)

=> http://www.gene-quantification.de/bioeps-courses-spring-summer-2009.pdf


Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


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