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<font face="Times New Roman, Times">Gene Quantification
Mailing 16. July 2002 <br>
<br>
NEW features on
</font><a href="http://www.wzw.tum.de/gene-quantification/" eudora="autourl"><font face="Times New Roman, Times" color="#0000FF"><u>http://www.wzw.tum.de/gene-quantification/<br>
</a></u></font>
<dl><font face="Symbol" size=4>
<dd>·<x-tab> </x-tab></font><font face="Times New Roman, Times">Housekeeping
Gene & Normalisation in kinetic PCR</font>
</dl><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">NEW
VERSIONS of REST© and REST-XL© <br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">NEW
POSTERS on Poster Boards<br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">Verification
of array results - NEW PUBLICATIONS<br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">real-time
PCR and RT-PCR - NEW PUBLICATIONS<br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">Endocrinology
- NEW PUBLICATIONS<br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">PCR,
RPA, Northern-Blot & Southern-Blot MOVIES<br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">Q-Gene
Software Processing of gene expression data generated
by quantitative real-time RT-PCR<br>
</font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times">TATAA
Biocenter: <b>q-PCR Network of Excellence<br>
</b></font><font face="Symbol" size=4>·<x-tab> </x-tab></font><font face="Times New Roman, Times"><b>NEW
real-time PCR primer and probe Database<br>
<br>
<br>
</b></font><font face="Times New Roman, Times" color="#0000FF"><u><a href="http://www.wzw.tum.de/gene-quantification/" eudora="autourl">http://www.wzw.tum.de/gene-quantification/<br>
<br>
<br>
</a></u></font><font face="Times New Roman, Times">Main focus of GENE
QUANTIFICATION web page is to describe<br>
and summarize all technical aspects involved in quantitative <br>
gene expression analysis using real-time PCR &
RT-PCR.<br>
( specificity, sensitivity, reproducibility, intra- & inter-assay
variations, <br>
kinetic PCR efficiency calculation, optimisation strategy, etc.)<br>
<br>
The GENE QUANTIFICATION web page illustrates the usefulness of a reliable
<br>
quantification strategy, and the difference between absolute vs. relative
quantification <br>
assays in kinetic PCR & kinetic RT-PCR.<br>
<br>
RT-PCR is the technique of choice for analysing mRNA in extremely low
abundance. <br>
Real-time RT-PCR using SYBR Green I detection combines the ease and
necessary <br>
exactness to be able to produce reliable as well as rapid results. <br>
To obtain high accuracy and reliability in RT and real-time PCR highly
defined <br>
quantification strategies and calculation methods are needed.<br>
<br>
For further questions contact Dr. Michael W. Pfaffl at<br>
</font><font face="Times New Roman, Times" color="#0000FF"><u>gene.quantification@wzw.tum.de<br>
<br>
</u></font><x-sigsep><p></x-sigsep>
<font color="#FF0000">********************************************************************<br>
</font><font size=5 color="#FF0000"><b>Gene Quantification @
wzw.tum.de<br>
<br>
</b></font><font color="#FF0000">e-mail:<x-tab> </x-tab><x-tab> </x-tab></font><font color="#0000FF">gene.quantification@wzw.tum.de
<br>
</font><font color="#FF0000">homepage</font><font color="#0000FF">:<x-tab> </x-tab><x-tab> </x-tab><a href="http://www.wzw.tum.de/gene-quantification/" eudora="autourl">http://www.wzw.tum.de/gene-quantification/</a><br>
<br>
</font><u>responsible Senior Scientist:<br>
</u>Dr. Michael W. Pfaffl<br>
Institute of Physiology<br>
Life Science Center Weihenstephan,<br>
Technical University of Munich<br>
Weihenstephaner Berg 3<br>
D-85350 Freising-Weihenstephan<br>
GERMANY<br>
<br>
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